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背景-Seq:CRISPR-Cas9针对性纳米孔测序用于抗菌素耐药性的传播动力学

Erica R Fuhrmeister^1,2, Sooyeol Kim², Shruteek A Mairal³

¹Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, Washington, USA.

bioRxiv : the preprint server for biology

|September 24, 2024

概括

抗菌素耐药性 (AMR) 在人类和动物之间传播. 一种新的方法,Context-Seq,追踪了像blaCTX-M和blaTEM这样的抗生素耐药性基因 (ARG),揭示了家庭中共享的AMR.

科学领域:

微生物学微生物学
基因组学就是基因组学.
一个健康,一个健康.

背景情况:

抗微生物药物耐药性 (AMR) 是一个重大的公共卫生威胁,需要在One Health框架内了解传播途径.
目前的测序方法在检测罕见抗生素耐药性基因 (ARG) 和跟踪其基因组背景或未培养物种之间的交换方面存在局限性.

研究的目的:

开发和验证一种新的有针对性的测序试验,Context-Seq,用于精确检测和描述ARG及其周围的遗传元素.
调查在肯尼亚内罗毕拥有动物的家庭中,人类,家禽和狗之间交换特定的AMR元素,blaCTX-M和blaTEM.

主要方法:

开发了Context-Seq,一种基于CRISPR-Cas9的定向测序试验,利用长期阅读的技术对ARG及其基因组背景进行选择性放大和测序.
应用了Context-Seq对拥有动物的家庭内人类和动物种群的样本,以识别和分析共享的AMR集群.

主要成果:

确定了22个不同的blaTEM遗传集群和一个blaCTX-M集群,在家庭内和家庭之间共享.
发现一半的AMR集群在人类和动物之间传播,另一半在动物之间传播.
检测到潜在的致病性细菌宿主,包括大肠杆菌和Klebsiella肺炎,在各种样本类型中携带ARG.

结论:

Context-Seq为研究AMR扩散和宿主动态的传统方法提供了一种强大的补充方法.
这些发现突出了家庭中AMR的显著物种间传播,强调了人类,动物和环境健康的相互联系.

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