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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jun 12, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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用PHOTON进行细胞下层空间转录学.

Shreya Rajachandran1,2, Qianlan Xu1,2, Qiqi Cao1,2

  • 1Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA.

bioRxiv : the preprint server for biology
|September 24, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了PHOTON (纳米尺度转录组的光选择),这是绘制细胞内RNA分布的新方法. 这种空间转录组工具揭示了RNA.

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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 基因组学就是基因组学.

背景情况:

  • 在亚细胞区内RNA的局部化对其功能至关重要,包括存储,处理,翻译和降解.
  • 了解RNA在精细分辨率上的空间分布对于破译RNA功能和调节机制至关重要.

研究的目的:

  • 介绍PHOTON (纳米尺度转录基因组的光选择),这是一种以亚细胞分辨率进行空间转录基因组分析的新方法.
  • 为了证明PHOTON在捕获特定细胞类型和亚细胞区内RNA的*in situ*转录组的能力.

主要方法:

  • PHOTON将高分辨率成像与高通量测序相结合.
  • 这种方法使得在亚细胞分辨率下进行空间转录组分析.

主要成果:

  • PHOTON准确地捕获目标细胞类型 (例如卵巢颗粒细胞) 的转录组 *in situ*.
  • 该方法分析了亚细胞区内的RNA含量,包括细胞核和压力颗粒.
  • "光子"揭示了m6A修饰在mRNA分裂成压力颗粒中的作用.

结论:

  • PHOTON是一个多功能和可通用的空间转录组分析平台.
  • 该方法提供了关于细胞下分子动力学和RNA调节的见解.