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相关概念视频

Proteomics01:33

Proteomics

7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

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相关实验视频

Updated: Jun 12, 2025

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
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参考样本的表观基因组,转录基因组和蛋白质基因组表征.

Chirag Nepal1,2, Wanqiu Chen1,2, Zhong Chen1,2

  • 1Center for Genomics, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.

bioRxiv : the preprint server for biology
|September 24, 2024
PubMed
概括
此摘要是机器生成的。

针对下一代测序技术,开发了采用包括表观基因组和蛋白质组在内的多种基因组数据的新参考标准. 这些标准有助于验证研究和临床应用的奥米克分析和生物信息学方法.

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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
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Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

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相关实验视频

Last Updated: Jun 12, 2025

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

3.4K
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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科学领域:

  • 基因组学和生物信息学
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 下一代测序 (NGS) 技术需要准确的参考标准用于研究和临床应用.
  • 之前的研究提供了基因组数据,但缺乏全面的表观基因组和蛋白质基因组特征.
  • 需要具有良好的特征的参考材料,集成多个omics数据类型.

研究的目的:

  • 开发综合基因组,表观基因组,转录基因组和蛋白质基因组数据的综合参考材料.
  • 描述表观基因组景观及其与转录组和蛋白质组的重叠.
  • 为验证欧米克分析和生物信息学方法提供标准.

主要方法:

  • 在两个细胞系上进行了ATAC-seq,甲基-seq,RNA-seq和蛋白质组分析.
  • 分析了体质突变,单核酸变异 (SNVs) 和结构变异 (SVs).
  • 研究了开放色素,DNA甲基化,CG密度和基因/蛋白质表达之间的关系.

主要成果:

  • 产生了表观基因组景观的全面目录,与转录组和蛋白质组重叠.
  • 鉴定了超过7,700种异型,大多数基因只有单个异型.
  • 观察到CpG岛屿 (CGI) 重叠的转录的蛋白质表达更高,并在突变中证明了SNVs.
  • 在CG丰富的区域中发现了低甲基化的开放色素区域,与更高的基因和蛋白质表达相关.
  • 在CG贫困地区确定了细胞系特异性甲基化和基因表达模式.

结论:

  • 开发了对两个细胞系具有多omics特征的精确定义的参考材料.
  • 这些材料作为验证和基准测试omics测试和生物信息学方法的有价值的标准.
  • 该资源将通过提高数据准确性和可重复性,使研究和临床社区受益.