相关实验视频

Updated: Jun 12, 2025

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models

Published on: March 24, 2019

开发阿尔茨海默病的诊断生物标志物,这些生物标志物基于与基因激素乳化相关的基因

Shaobo Guo^1,2,3, Wenhui Zhu^1,2,3, Yuting Bian^1,3

¹The Affiliated Hospital of Nanjing University of Chinese Medicine, Department of Geriatric, Nanjing, China.

|September 24, 2024

概括

相关的基因 (ARID5B,SESN1,XPA) 显示出潜在的阿尔茨海默病 (AD) 生物标志物. 这些基因与AD进展和免疫细胞相互作用有关,提供了新的诊断途径.

科学领域:

生物化学和分子生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

基斯乳化在阿尔茨海默氏病 (AD) 发病过程中起作用.
在阿尔茨海默病中,与基因激素乳化相关的基因 (HLRGs) 的分子机制需要进一步研究.

研究的目的:

识别和验证与胰岛素乳化相关的基因 (HLRGs) 作为阿尔茨海默病 (AD) 的潜在生物标志物.
探索与这些生物标志物相关的分子机制,免疫细胞协会和治疗点.

主要方法:

权重基因共同表达网络分析 (WGCNA) 和差异基因表达分析被用于识别候选基因.
机器学习和ROC曲线分析验证了诊断基因,而GSEA探索了分子通路.
分析了AD模型小鼠大脑中的ceRNA网络,药物标和基因表达.

主要成果:

确定了五个候选基因 (ARID5B,NSMCE4A,SESN1,THADA,XPA),富含嗅觉转导和N-甘氨酸生物合成途径.
在诊断基因和天真B细胞之间发现了强烈的正相关性.
三个基因 (ARID5B,SESN1,XPA) 被验证为AD生物标志物,在AD模型小鼠大脑中显示上调表达.

结论:

在阿尔茨海默病中,ARID5B,SESN1和XPA被确定为潜在的生物标志物,与胰岛素乳糖化有关.
这些生物标志物与阿尔茨海默病的进展有着强烈的关联,并可能提供新的诊断和治疗见解.

关键词:

阿尔茨海默病的疾病阿尔茨海默病的疾病.生物标志物生物标志物诊断诊断诊断诊断诊断相关的基因基因相关的基因基因相关的基因机器学习是机器学习.

更多相关视频

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Published on: May 20, 2024

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

相关概念视频

Alzheimer's Disease: Overview

Alzheimer's Disease: Overview

Alzheimer's Disease (AD) is a continually advancing neurodegenerative disorder, distinguished by escalating memory loss, cognitive dysfunction, and dementia. The disease unfolds in three stages: preclinical, mild cognitive impairment (MCI), and dementia. Its onset is insidious, and the progression gradual, with the cause not well explained by other disorders.
The clinical diagnosis of AD hinges on the presence of memory and other cognitive impairments. Biomarkers, such as changes in Aβ...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...