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在手指小米中,核因子-Y (NF-Y) 转录因子家族的全基因组鉴定揭示了结构和功能多样性

Varsha Rani^1,2, Vinay Kumar Singh³, D C Joshi⁴

¹Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, 273009, Uttar Pradesh, India.

|September 24, 2024

概括

这项研究确定了手指小米中57个Y核因子 (NF-Y) 基因,揭示了它们在承受压力的潜在作用. 这些基因提供了改善这种耐受性作物的目标,以抵御生物和非生物压力.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

核因子Y (NF-Y) 转录因子调节压力反应和发育.
指小米 (Eleusine coracana) 是一种耐压作物,具有较差的NF-Y基因特征.
了解指小米的NF-Y对于作物改进至关重要.

研究的目的:

使用生物信息学识别和描述指小米中的NF-Y基因.
探索 EcNF-Y 基因在应激耐受性中的潜在作用.

主要方法:

在指小米中全基因组挖掘NF-Y基因.
对基因组织,保存的动机,cis调节元素和miRNA点位点的分析.
核酸替代率和基因重复分析.

主要成果:

确定了57个EcNF-Y基因 (18个NF-YA,23个NF-YB,16个NF-YC).
基因复制分析显示了7个NF-YA,10个NF-YB和8个NF-YC对应物.
存在应激反应的 cis 元素 (ABRE,DRE,MYB/MYC) 和 miR169 向 EcNF-YA13.

结论:

EcNF-Y基因是指小米中非生物应激耐受性的潜在调节者.
miR169-EcNF-YA13相互作用可能是改善爆发性真菌耐药性的目标.
这项研究为探索生物和非生物压力耐受性中的EcNF-Y功能提供了基础.

关键词:

无生物应激应激是一种非生物应激.指小米是指小米中的一种.马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.核因子-Y 核因子-Y 转录因子是一种转录因子.这是一个小RNARNA.

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