相关实验视频

Updated: Jun 12, 2025

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

使用标准与拉普拉斯-哈明二元化方法进行HR-pQCT交叉校准

Saghi Sadoughi¹, Aditya Subramanian¹, Gabriella Ramil¹

¹Bone Quality Research Lab, Department of Radiology and Biomedical Imaging, University of California, San Francisco, 185 Berry St., Suite 350, San Francisco, CA 94107, United States.

|September 24, 2024

概括

交叉校准高分辨率外围定量计算断层扫描仪 (HR-pQCT) 扫描仪对于比较骨微架构数据至关重要. 这项研究开发了方程,从第一代 (XCTI) 数据中估计第二代 (XCTII) 测量,从而提高了数据的可比性.

科学领域:

* * 医学影像成像
* 骨生物学骨生物学
* 生物医学工程 * 生物医学工程

背景情况:

*高分辨率的外围定量计算断层扫描 (HR-pQCT) 对于评估骨微架构至关重要.
* HR-pQCT扫描器代 (XCTI到XCTII) 之间的过渡使纵向和多中心研究复杂化.
* 交叉校准对于整合不同HR-pQCT设备的数据至关重要.

研究的目的:

* 建立交叉校准方程,用XCTI数据来估计XCTII HR-pQCT测量结果.
* 在与XCTI进行交叉校准时,将XCTII数据的标准与拉普拉斯-哈明 (LH) 二元化进行比较.
* 为了方便对历史HR-pQCT骨数据的比较和利用.

主要方法:

* 在XCTI和XCTIIHR-pQCT扫描仪上扫描了36名志愿者的半径和骨.
*使用标准协议分析了XCTI图像.
*使用标准和拉普拉斯-哈明 (LH) 二元化方法分析了XCTII图像.
*使用线性回归来推导交叉校准方程.

主要成果:

* XCTI和XCTII之间在骨密度和几何结果方面发现了强烈的相关性.
* 尽管绝对值有差异,但大多数微观结构结果显示出强烈的相关性.
*与XCTI数据相比,XCTII的LH二元化方法改善了与标准XCTII协议相比的相关性,并减少了与XCTI数据相比的分辨率敏感指标的偏差.

结论:

*交叉校准方程允许从XCTI数据估计XCTII测量结果.
* 建议使用拉普拉斯-哈明 (LH) 二元化方法进行XCTII分析,特别是与XCTI数据进行比较.
*使用LH方法可以提高跨HR-pQCT扫描器代的骨微架构数据的准确性和可比性.

关键词:

高斯二进制化的高斯二进制化人力资源-pQCTCT 在拉普拉斯 - 汉明二元化进行交叉校准.估计错误的估计错误

更多相关视频

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

相关概念视频

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

2D NMR: Overview of Heteronuclear Correlation Techniques

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...