微塑料诱导的阴茎损伤:形态和免疫组织化学研究
Shaimaa M M Saleh1, Souzan Abdel-Zaher2, Mahmoud S Mohamed1
1Department of Zoology, Faculty of Science, Assiut University, Assiut, Egypt.
Microscopy research and technique
|September 24, 2024
概括
暴露于聚乙烯微塑料 (MPs) 造成了哺乳动物大皮的显著损伤,包括细胞变化和组织变形. 恢复期有助于改善大多数这些不良影响.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 胃肠病学 胃肠病学
背景情况:
- 微塑料 (MP),特别是聚乙烯 (PE),是普遍存在的环境污染物.
- 虽然研究了与其他化学物质的结合作用,但单次使用的PE MPs对哺乳动物皮的影响仍未得到充分研究.
研究的目的:
- 为了研究聚乙烯微塑料 (PE MP) 暴露在C57BL/6小鼠的皮质上对细胞水平的影响.
- 评估不同PE MP度和随后的恢复期对肠道组织的影响.
主要方法:
- 小鼠被暴露在不同度的PE MPs (6,60,600μg/mL) 中15天,随后有15天的恢复期.
- 组织学,组织化学 (Alcian蓝色,PAS),免疫组织化学 (P53,Ki-67) 和传输电子显微镜被用来分析阴茎组织.
主要成果:
- 暴露于PE MP诱导了核皮克诺斯,形变形,膜自身退化和杯状细胞增生,分泌量增加.
- 观察到P53和Ki-67表达的显著上调,特别是在MP度较高时.
- 超结构分析证实了细胞内的MP沉积.
结论:
- 单次使用的聚乙烯微塑料会对哺乳动物的皮质造成显著的形态和细胞损伤.
- 恢复期可以在很大程度上扭转观察到的变化,这表明肠道愈合的潜力.
- 需要进一步的研究来了解摄入微塑料的长期影响.
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