应用不同的染色方法在色素丰富的黑色素瘤的诊断
Zhou Chengmin1, Li Jiayu1, Hou Jun1
1Department of Pathology, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Journal of clinical laboratory analysis
|September 24, 2024
概括
对于诊断具有重色素干扰的黑色素瘤, permanganate 脱色,其次是性酸盐红色 (AP红色) 染色或多重免疫光学 (mIF) 提供了优异的结果. 建议使用AP红色,因为它具有成本效益和实用性.
科学领域:
- 皮肤病理学 皮肤病理学
- 免疫组织化学 免疫组织化学
- 在瘤学瘤学.
背景情况:
- 黑色素瘤的诊断可能是具有挑战性的,因为严重的色素干扰在免疫组织化学解释.
- 有效的脱色方法对于准确的染色和生物标志物检测至关重要.
研究的目的:
- 为了比较不同的脱色和染色技术,以诊断黑色素瘤与显著的色素干扰.
- 在具有挑战性的情况下,确定最佳方法来改善免疫组织化学解释.
主要方法:
- 黑色素瘤组织使用高酸 (0.1%-1%) 进行了脱色,持续时间不同 (1-240分钟).
- 脱色组织被染色使用二氨二丁 (DAB),性酸酶快红色 (AP红色),多重免疫光 (mIF),3-氨基-9-乙基醇 (AEC) 和硫酸铁.
- 评估了黑色素瘤标记物 (HMB45,MelanA,S100,SOX10) 和扩散标记物 (ki67) 的积极表达.
主要成果:
- 用0.5%的 permanganate 在15分钟内实现了最佳的脱色.
- 脱色后的抗体阳性率有所不同:DAB (41.7%-66.7%),AP红 (66.7%-91.7%),mIF (83.3%-100%),AEC (25%-33.3%) 和硫酸铁 (33.3%).
- 多重免疫光和AP红色染色显示出最高的阳性率.
结论:
- 性酸酶红色 (AP红色) 染色和多重免疫光 (mIF) 是非常适合诊断黑色素瘤严重的色素干扰.
- 建议使用AP红色染色作为例行诊断的更经济和实用的选择.
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