使用发射细菌杀菌性紫外线和抗微生物蓝光的LED系统来使生物膜失活
Dana Pousty1, Ben Ma2, Christian Mathews3
1School of Mechanical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel.
Water research
|September 24, 2024
概括
杀菌性紫外线 (UV) 和抗微生物蓝光 (aBL) 有效地使常见材料上的水borne生物膜失活. 紫外线在较低的流量下工作,而aBL需要更高的剂量,性能因表面特性而异.
科学领域:
- 环境微生物学 环境微生物学
- 水处理技术水处理技术
- 消毒科学 消毒科学
背景情况:
- 水系统中的生物薄膜通过庇护病原体,对饮用水安全构成重大风险.
- 寻求非化学消毒方法作为传统水处理的替代方法.
- 以光为基础的消毒,包括杀菌性紫外线 (UV) 和抗微生物蓝光 (aBL),对生物膜控制有希望.
研究的目的:
- 研究紫外线和aBL在灭活Pseudomonas aeruginosa和混合培养生物膜中的有效性.
- 为了比较低压 (LP) 紫外线灯 (254 nm),紫外线发光二极管 (LED) (270 nm) 和aBL LED (405 nm) 的性能.
- 评估表面材料特性 (聚碳酸,PTFE,PVC) 对基于光的生物膜失活的影响.
主要方法:
- 在聚碳酸,PTFE和PVC材料上开发纯培养和混合培养生物膜.
- 生物膜暴露于三个不同的光源:LP紫外灯,UV LED和aBL LED.
- 基于光流和材料表面特征的生物膜失活量的量化.
主要成果:
- 所有测试的光照装置都显示出对纯培养和混合培养生物膜的有效失活.
- 紫外线设备 (LP UV和UV LED) 在较低的流量下实现了显著的无活化 (在20mJ/cm2时>1日志减少).
- 对于纯培养生物膜的实质性失活,aBL LED 需要显著更高的流动量 (在 >200,000 mJ/cm2 时最大3.8 ± 0.5 个日志减少).
- 生物膜无活化在不同的表面材料中有所不同,受到粗性,疏水性和表面电荷等特性的影响.
结论:
- 基于光的消毒方法对于控制水系统中的生物膜是有效的.
- 与aBL相比,紫外线光在较低的能量剂量下提供有效的无活化.
- 表面材料的特性在基于光的生物膜消毒的有效性中起着至关重要的作用,需要考虑特定材料的处理.
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