为直接评估后生物的功能而建立一个形外有机模型
Yeonoh Cho1, Moon-Hee Sung2, Hee-Taik Kang3
1Department of Food Science and Biotechnology, College of Bio-Nano Technology, Gachon University, Gyeonggi 13120, Republic of Korea.
Journal of microbiology and biotechnology
|September 24, 2024
概括
新的尖端外肠道有机体揭示了后生物质保护肠道屏障功能. 这种3D模型表明,来自特定细菌的后生菌可以增强紧密的结节和线粒体健康,防止脂多糖 (LPS) 损伤.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 胃肠病学 胃肠病学
背景情况:
- 在体外有机体为研究器官生理学提供了一个3D模型系统,克服了2D培养的局限性.
- 评估后生菌对肠道屏障的功能影响需要允许与顶端表面直接相互作用的模型.
研究的目的:
- 评估后生物治疗对肠道有机体的疗效,使用角外模型.
- 为了研究后生物质对脂聚糖 (LPS) 诱导的损伤,紧密结合完整性和线粒体平衡的影响.
主要方法:
- 开发和使用一个顶部外肠道有机体模型.
- 用来自*Lactiplantibacillus plantarum* KM2和*Bacillus velezensis* KMU01的复合超剂对有机体进行处理,作为后生物.
- 评估循环氧化酶-2 (COX-2) 表达,紧接标记表达 (ZO-1,克劳丁,奥克卢丁) 和线粒体平衡因子 (PINK1,帕金,PGC1a).
主要成果:
- 后生物治疗显著降低了LPS挑战器官中的COX-2表达.
- 紧结蛋白 (ZO-1,克劳丁,奥克卢丁) 的表达因后生物药物而增加.
- 线粒体平衡因子 (PINK1,parkin,PGC1a) 的表达被上调,这表明线粒体功能和线粒体的增强.
结论:
- 顶部外肠道有机体模型对于评估后生物功能是有效的.
- 后生物保护肠道紧接口免受LPS诱导的损伤.
- 后生物质通过线粒体和生物生成维持线粒体平衡,这表明它在肠道健康中起着作用.
相关概念视频
01:20Pharmacokinetic Models: Overview
Pharmacokinetic models utilize mathematical analysis to achieve a detailed quantitative understanding of a drug's life cycle within the body. They are instrumental in simulating a drug's pharmacokinetic parameters, predicting drug concentrations over time, optimizing dosage regimens, linking concentrations with pharmacologic activity, and estimating potential toxicity.
There are three primary types of models: empirical, compartment, and physiological. Empirical models, with minimal assumptions,...
There are three primary types of models: empirical, compartment, and physiological. Empirical models, with minimal assumptions,...
01:26Pharmacokinetic Models: Comparison and Selection Criterion
Physiological and compartmental models are valuable tools used in studying biological systems. These models rely on differential equations to maintain mass balance within the system, ensuring an accurate representation of the dynamic processes at play.
Physiological models take a detailed approach by considering specific molecular processes. They can predict drug distribution, metabolism, and elimination changes, providing a comprehensive understanding of how drugs interact with the body.
Physiological models take a detailed approach by considering specific molecular processes. They can predict drug distribution, metabolism, and elimination changes, providing a comprehensive understanding of how drugs interact with the body.