相关实验视频

Updated: Jun 12, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

开发和应用GenoBaits WheatSNP16K阵列,以加速小麦遗传研究和育种

Shengjie Liu¹, Mingjie Xiang², Xiaoting Wang²

¹State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.

Plant communications

|September 25, 2024

概括

一个新的基因型阵列 (GBW16K) 为小麦遗传分析提供了一个具有成本效益的工具. 它成功地识别了条纹生抗性基因,有助于培育耐久性疾病控制.

科学领域:

植物遗传学植物遗传学
分子育种是指分子育种.
农业科学农业科学

背景情况:

单核酸多态 (SNP) 是小麦遗传研究的关键分子标记.
现有的SNP基因型识别平台可能是苛刻且昂贵的.
通过液晶芯片技术进行目标测序 (GBTS) 的基因型定型提供了更容易获得的替代方案.

研究的目的:

设计和评估一个新的GenoBaits WheatSNP16K (GBW16K) 基因造型阵列.
评估该阵列对遗传链接映射和在小麦中识别疾病抵抗基因的实用性.

主要方法:

使用现有的小麦SNP和重新排序数据设计了GBW16K GBTS阵列.
基因定型了239种不同的小麦加入,以分析遗传多样性和人口结构.
使用复合同血统种群构建了一个遗传链接地图.

主要成果:

GBW16K 数组包含 14,868 个目标 SNP 区域,在 239 个加入中识别了 37,669 个 SNP.
遗传多样性的分析证实了该小组适合绘制地图.
鉴定了带状耐基因 (Yr27, Yr30, QYr.nwafu-2BL.4, Yr18) 并用候选基因对QYr.nwafu-2BL.4进行了表征.

结论:

GBW16K 阵列是用于小麦遗传分析和育种的有价值,用户友好的工具.
已识别的条纹生抗性基因有助于开发持久的抗病策略.
这项技术有助于在小麦中有效地进行基因映射和基因发现.

关键词:

通过目标测序进行基因型鉴定.液体阵列是一种液体阵列.耐药性基因的基因是耐药性的基因.条纹生条纹生小麦小麦小麦小麦小麦小麦小麦.

更多相关视频

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

Published on: November 30, 2006

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

相关概念视频

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

The Central Dogma

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...