基于比较转录组学的红酵母大米中的颜料和氨酸生物合成上阿斯科布酸的影响研究
Zhilin Jiang1,2, Yunxun Duan1,2, Qianqian Yin2
1Key Laboratory of the Evaluation and Monitoring of Southwest Land Resources (Ministry of Education), Sichuan Normal University, Chengdu, Sichuan, China.
Frontiers in microbiology
|September 25, 2024
概括
亚酸 (EAA) 显著增强红酵母大米颜料的生产,同时降低有害的氨酸水平. 这项研究揭示了参与这些有益变化的关键代谢途径和基因.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 代谢学 代谢学 代谢学
背景情况:
- 红酵母大米因其颜料而受到重视,但氨酸污染构成了食品安全风险.
- 了解新陈代谢调节对于改善红酵母大米质量至关重要.
研究的目的:
- 为了研究 Askorbic 酸 (EAA) 对红酵母大米的色素和素水平的影响.
- 通过转录学解释这些变化背后的代谢机制.
主要方法:
- 进行比较的转录学分析.
- 测量颜料和氨酸含量.
- 对酸蛋白酶活性,DPPH清除率和总降解能力的测定.
主要成果:
- EAA增加了58.2%的色素,并减少了65.4%的氨酸.
- EAA增强了酸蛋白酶活性,DPPH清理率和总的降解能力.
- 确定了791个差异表达的基因和79个代谢途径,包括碳,氨基酸和脂肪酸代谢.
- 特定的基因 (例如,M_pigC,M_pigE,M_pigH,M_pigN,ctnC,pksCT) 与色素和氨酸调节有关.
结论:
- 亚斯科布酸通过增强色素和减少氨酸,有效地改善红酵母大米的质量.
- 转录组分析提供了EAA对红酵母大米代谢影响的分子基础的见解.
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