机构间实验室标准化用于SARS-CoV-2通过废水流行病学监测,应用于墨西哥城的废水流行病学
Mariel Araceli Oyervides-Muñoz1,2, Alberto Aguayo-Acosta1,2, Daniel de Los Cobos-Vasconcelos3
1Tecnologico de Monterrey, Institute of Advanced Materials for Sustainable Manufacturing, Monterrey, Mexico.
IJID regions
|September 25, 2024
概括
在墨西哥城对SARS-CoV-2的废水监测标准化使得可靠的COVID-19监测成为可能. 这项实验室间研究建立了一个统一的协议,用于在多个实验室中准确量化病毒载量.
科学领域:
- 环境微生物学环境微生物学
- 流行病学 流行病学
- 公共卫生 公共卫生
背景情况:
- 基于废水的监测 (WBS) 对COVID-19的流行病学预防至关重要.
- 为了获得可靠的WBS结果,需要进行标准化,特别是在大城市.
研究的目的:
- 为废水中SARS-CoV-2量化建立一个单一的,标准化的协议.
- 确保墨西哥多个实验室的WBS数据可比.
主要方法:
- 这是一项涉及七个实验室 (六个在墨西哥,一个在美国) 的实验室间研究.
- 考虑成本,基础设施和材料可用性的协议的开发.
- 使用循环值和MS2菌体恢复率进行SARS-CoV-2量化分析.
主要成果:
- 布兰德-阿尔特曼分析显示量化的一致性,在两个实验室中存在小差异.
- MS2菌的恢复率从35%到67%不等.
- 病毒遗传物质的恢复效率在65%至93%之间.
结论:
- 该研究为墨西哥持续和广泛的WBS提供了基础.
- 标准化的协议促进了时间和资源的有效监控.
- 这种方法支持独立实验室用于公共卫生决策的应用.
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