长寿的schistosoma mansoni循环的阴极抗原在纸干尿斑点的过器
Abdallah Zacharia1, Clemence Kinabo1, Twilumba Makene1
1Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania.
Practical laboratory medicine
|September 25, 2024
概括
在干燥的尿样中仍然可以检测到Schistosoma mansoni循环的正极抗原 (CCA),即使在不同温度下经过12周. 这种稳定性支持使用基于过纸的干尿斑用于Schistosoma mansoni诊断.
科学领域:
- 寄生虫学的寄生虫学
- 热带医学 热带医学
- 生物化学 生物化学
背景情况:
- 顺病的诊断依赖于检测寄生虫抗原.
- 循环阴极抗原 (CCA) 是schistosoma mansoni感染的关键生物标志物.
- 评估存储样本中的诊断标记物的稳定性对于现场应用至关重要.
研究的目的:
- 为了评估Schistosoma mansoni循环阴极抗原 (CCA) 在基于过纸的干尿斑点 (FP-DUS) 的时间稳定性.
- 确定不同储存温度 (室温,4°C,-20°C) 对12周内尿液和FP-DUS样品中CCA检测能力的影响.
主要方法:
- 从20名确诊感染S. mansoni的儿童收集了尿液样本.
- 样品以液体尿液和FP-DUS的形式在室温,4°C和-20°C下存储.
- 在4,8和12周使用Schisto POC-CCA测定来评估CCA水平.
主要成果:
- 在FP-DUS样本中,CCA得分最初增加,然后强度下降,但无论温度如何,仍然为正值.
- 超过80%的尿样在4至12周保持了POC-CCA得分3+的水平.
- 两个室温液体尿样在第8周和第12周呈阴性结果.
结论:
- 在FP-DUS样本中,schistosoma mansoni CCA表现出良好的时间稳定性.
- FP-DUS提供了一种可行的方法来存储尿样用于S. mansoni诊断,保持标记物的检测能力.
- 这一发现支持FP-DUS的潜力,用于分散和长期存储的诊断样本在杆菌瘤的监测计划.
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