ADCY5作为质母细胞瘤的假定瘤抑制剂:综合分析
Wang Can1, Wen Yan2, Huang Luo1
1Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 400030, China.
Heliyon
|September 25, 2024
概括
基酶5 (ADCY5) 在质母细胞瘤 (GBM) 中降低调节,并与更差的预后有关. 恢复ADCY5抑制了GBM细胞的生长和入侵,表明其作为生物标志物和治疗点的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 腺环酶 (AC) 异型因癌症而存在.
- 在质母细胞瘤 (GBM) 中ADCY5的作用仍然未被描述.
- 这项研究调查了ADCY5表达,表观遗传改变和GBM的预后意义.
研究的目的:
- 确定ADCY5在GBM中的表达特征.
- 分析ADCY5在GBM中的预后价值和表观遗传调节.
- 阐明ADCY5在GBM细胞行为中的功能作用.
主要方法:
- 公开数据集的生物信息分析 (GEPIA,HPA,Kaplan-Meier Plotter,CGGA,DiseaseMeth,cBioPortal,STRING).这些数据集中的生物信息分析方法包括:
- 在体外实验包括RT-PCR,CCK-8,殖民地形成,伤口愈合和Transwell测试.
- 对ADCY5联合表达基因和相关信号通路 (cAMP/AKT,cGMP-PKG,Wnts) 的分析.
主要成果:
- 在GBM组织和细胞系中,ADCY5mRNA和蛋白质的表达下调.
- ADCY5表达与DNA甲基化有负相关性,并与临床参数相关.
- 高ADCY5表达作为GBM的独立预后因素.
- ADCY5的再表达抑制了GBM细胞的增殖,迁移,入侵和上皮-介质细胞过渡 (EMT).
结论:
- 在GBM中,ADCY5作为潜在的表观遗传生物标志物起作用.
- ADCY5对GBM患者来说是一个有前途的预后标.
相关概念视频
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
01:12Loss of Tumor Suppressor Gene Functions
4.7K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.7K
02:07Tumor Progression
6.3K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.3K
02:53Adaptive Mechanisms in Cancer Cells
5.7K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.7K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:27Tumor Immunotherapy
491
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
491