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酵母STE12产品是表达两组细胞类型特定基因的必要条件

S Fields, I Herskowitz

|October 1, 1985

概括

研究人员确定了STE12基因产物作为酵母交配中的alpha特异性和a特异性基因的关键激活剂. 这一发现揭示了STE12的存在.

科学领域:

分子生物学分子生物学
酵母遗传学酵母遗传学
细胞分化细胞分化

背景情况:

酵母细胞 (alpha和a) 表达不同的基因进行交配.
阿尔法1蛋白 (来自MAT阿尔法位) 是这些细胞类型特定基因的唯一已知的激活剂.
阿尔法特异性基因包括主要的阿尔法因子基因,对交配至关重要.

研究的目的:

为了确定阿尔法特异性基因表达的新调节者.
调查已识别的基因在酵母细胞类型专业化中的作用.

主要方法:

查酵母突变体不能表达主要的α因子基因.
在已识别的突变菌株中分析RNA的产生 (ste12).
在野生型和ste12突变酵母细胞中比较基因表达模式.

主要成果:

鉴定出STE12基因产物是α-因子基因的积极调节者.
ste12突变体显示出来自其他α特异基因的RNA生产缺陷.
同时,ste12突变者对特定基因的RNA水平也有所降低.

结论:

STE12基因产物是一种新型激活剂,用于酵母中的α特异性和a特异性基因.
STE12在酵母细胞类型的专业化和交配行为中起着至关重要的作用.
这一发现扩大了对酵母分化的转录调节的理解.

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