[振荡静态循环培养增强了Ganoderma lucidum中三烯和菌体活动的合成]
Xingyi Jiang1,2, Wei Han2, Jia Guo1
1Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|September 25, 2024
概括
这项研究优化了Ganoderma lucidum的液态发酵,显著增加了三烯的产量. 新方法提高了产量,缩短了种植时间,为生产有价值的真菌化合物提供了更有效的方法.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 药用真菌Ganoderma lucidum含有对其生物活性负责的三烯.
- 传统的G. lucidum发酵方法在效率和产量方面存在局限性.
- 优化三烯生产对于利用G. lucidum的治疗潜力至关重要.
研究的目的:
- 开发一种高效的液态发酵技术,以提高 Ganoderma lucidum 的三烯产量.
- 为了克服传统的潜水和两阶段培养方法的局限性.
- 使用人工神经网络和遗传算法优化种植条件.
主要方法:
- 建立了Ganoderma lucidum的振荡静态循环培养过程.
- 利用遗传算法和人工神经网络来优化发酵参数.
- 确定了高产量三烯生产的最佳振荡和静态培养持续时间.
主要成果:
- 获得了20.82 mg/g的三烯含量和129.09 mg/L的产量,比Z10J0方法增加了324.78%.
- 将整体种植周期缩短了10.6天.
- 培养的菌体显示出显著的抗瘤和抗氧化活性.
结论:
- 开发的振荡静态循环培养是一种经济高效的液态发酵方法,用于高产三烯生产.
- 这种方法简化了发酵,缩短了发酵时间,克服了传统技术的缺点.
- 这些发现为液态发酵在Ganoderma lucidum三烯生产中的规模应用提供了宝贵的见解.
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