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磁性粒子喷雾质谱法用于在等离子体样本中测定β抑制剂

Elizângela Maria de Castilho Alves Morais¹, Tássia Venga Mendes¹, Estela Domingos Arnoni¹

¹Laboratory of Toxicant and Drug Analyses, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, MG, 37130-001, Brazil.

Mikrochimica acta

|September 25, 2024

概括

磁粒子喷雾质谱法 (MPS-MS) 提供了一种快速,灵敏的方法来检测等离子体中的β抑制剂. 这种创新技术结合了样品制备和环境电离,与绿色化学原则保持一致.

科学领域:

分析化学分析化学
生物医学科学生物医学科学

背景情况:

β抑制剂是重要的心血管药物.
在等离子体等生物矩阵中准确量化对于治疗药物监测至关重要.
传统方法往往涉及复杂的样本准备,可能耗时.

研究的目的:

开发和验证一种用于在人体血中测定β抑制剂的新方法.
使用磁粒子喷雾质谱法 (MPS-MS) 进行快速和敏感的分析.
与现有技术相比,评估MPS-MS的性能和优势.

主要方法:

合成了磁性分子印记聚合物 (M-MIP) 颗粒,并用于分散固相提取.
用oxprenolol作为选择性β-阻断剂捕获的伪模板来功能化M-MIP粒子.
使用MPS-MS与三重四极质谱仪相结合,进行了分析物的脱吸,电离和检测.

主要成果:

开发的MPS-MS方法对β抑制剂具有良好的选择性.
线性分析曲线 (R2>0.98) 在量化极限为3μg L−1.1.时得到.
观察到高灵敏度,检测极限在0.64至2.41μg L-1.1之间.
获得了精确和准确的结果,相对标准偏差和相对误差在可接受的范围内.

结论:

MPS-MS是一种简单,敏感和快速的技术,用于在人体血中测定β阻断剂.
该方法消除了对色谱分离的需要,并避免了离子抑制.
MPS-MS为传统分析方法提供了一个有利的,与绿色化学一致的替代方案.

关键词:

环境的电离化环境.分散固体相提取方法分散固体相提取方法磁性粒子喷雾质谱学质谱学磁吸剂是一种磁吸剂.有分子印记的聚合物.

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