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Updated: May 9, 2026

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一种可靠的qPCR技术用于检测可行的Xanthomonas arboricola pv. 修剪细胞 修剪细胞

Pilar Sabuquillo1, Isabel M Berruete2, Jaime Cubero3

  • 1Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain. mpsc@inia.csic.es.

Applied microbiology and biotechnology
|September 25, 2024
PubMed
概括

一种新的可行的定量PCR (v-qPCR) 方法只能准确地检测活的Xanthomonas arboricola pv. pruni (Xap) 的细菌细胞. 这种方法可以防止过度估计感染,减少石头水果和杏仁作物不必要的化学处理.

关键词:
桑托蒙纳斯树木植物植物 pv. 普鲁尼普鲁尼 (Pruni Pruni) 是一个切断线 Ct 的位置.间隙性染料是一种染料.这是一个 LOD LOD LOD.这就是V-qPCRR.可活性的细胞检测检测.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...