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Updated: Jun 12, 2025

Minimum Volume Vitrification of Immature Feline Oocytes
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使用基于微流体芯片的自动设备进行卵细胞玻璃化.

Jing Shen1,2,3, Zixuan Yu1, Weijie Li1,2,3

  • 1Institute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Annals of biomedical engineering
|September 25, 2024
PubMed
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与手工方法相比,新的基于微流体的自动化玻璃化 (MAV) 装置显著改善了卵细胞存活率和发育率. 在MAV系统中优化了CPA输送协议,进一步提高了维护生育能力的玻璃化成功.

科学领域:

  • 辅助生殖技术 辅助生殖技术
  • 低温生物学 低温生物学
  • 微流体学 微流体学

背景情况:

  • 卵细胞玻璃化对于辅助生殖和生育能力的保护至关重要.
  • 手动冷保护剂 (CPA) 操纵有透冲击的风险,需要专家技能.
  • 需要自动化系统来标准化和改善玻璃化结果.

研究的目的:

  • 开发和评估基于微流体的自动化玻璃化 (MAV) 装置,用于卵细胞冷保存.
  • 为了比较MAV与手动Cryotop/QC方法的疗效.
  • 在MAV系统中优化CPA加载和删除协议.

主要方法:

  • 一个微流体系统被设计为精确的,线性CPA度调整.
  • 为了自动化,MAV设备将CPA处理与玻璃化过程集成在一起.
  • 卵子被使用MAV与不同的CPA输送段 (1,2,4) 进行玻璃化,并与手动Cryotop/QC进行比较.

主要成果:

  • 比手工方法 (73.35%,43.73%,23.67%),MAV方法的产卵细胞存活率 (80.44%),裂变率 (54.17%) 和胚胎细胞发育率 (32.95%) 显著更高.
  • 使用4段CPA输送协议进行玻璃化卵细胞显示出优异的结果:86.18%的生存率,63.29%的裂变率和45.58%的胚芽细胞率.
关键词:
自动化玻璃化自动化玻璃化微流体学 微流体学卵细胞是卵细胞中的一个.透性损伤是指透性损伤.

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  • 特定的CPA度协议 (VS1-TS1) 显示了最高的回暖生存率,而其他 (VS3-TS3) 显示了激活后更高的胚胎细胞率.

    • 结论:

    • MAV设备为卵细胞玻璃化提供了一个有前途的自动化解决方案,提高了成功率.
    • 优化的多段CPA输送协议提高了卵细胞冷保存的结果.
    • 这项技术在促进生育保护和辅助生殖方面具有重大潜力.