基于QuEChERS的多种残留物分析方法,其次是超高性能液态染色学,再加上二极管阵列检测器,检测人类血清和母乳中的农药
Prapha Arnnok1, Rodjana Burakham2
1Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand.
Bioanalysis
|September 25, 2024
概括
经过验证的方法可以检测母亲血清和母乳中的农药,这对于评估早期暴露风险至关重要. 在配对样本中发现了6种农药.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 孕产妇的流体对于评估早期的农药暴露至关重要.
- 杀虫剂可以通过产前暴露和哺乳转移到新生儿身上.
- 了解农药转移对于婴儿健康风险评估至关重要.
研究的目的:
- 为验证人类血清和母乳的UHPLC-DAD和修改后的QuEChERS方法.
- 评估生物样本对农药检测的矩阵效应.
- 在配对的母体样本中应用验证的方法检测农药.
主要方法:
- 开发并验证了使用二极管阵列检测 (UHPLC-DAD) 的超高性能液态染色学.
- 修改QuEChERS (快速,轻松,廉价,坚固和安全) 提取血清和母乳的提取方法.
- 对母乳样本进行评估的矩阵效应和实施的脂质去除.
主要成果:
- 全面验证了UHPLC-DAD和修改后的QuEChERS方法.
- 观察到显著的矩阵效应,特别是对低敏感性农药.
- 血清和母乳提取方案得到了优化,包括添加河和去除牛奶中的脂质.
结论:
- 经过验证的UHPLC-DAD和修改的QuEChERS方法是可靠的,用于对母体液体中的农药分析.
- 该研究成功地在配对的人类血清和母乳样本中确定了6种农药.
- 这些发现有助于了解早期生活中的农药暴露途径.
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