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光场断层扫描光终身成像显微镜

Yayao Ma¹, Jongchan Park¹, Luzhe Huang^1,2,3

¹Department of Bioengineering, University of California, Los Angeles, CA 90095.

Proceedings of the National Academy of Sciences of the United States of America

|September 25, 2024

概括

我们开发了光场断层成像FLIM (LIFT-FLIM),这是一种计算成像技术,可以显著减少扫描步骤,以实现更快,高分辨率的3D光终身成像显微镜. 这一突破增强了分子水平的生物可视化.

科学领域:

生物光学和成像科学生物光学和成像科学
分子和细胞生物学分子和细胞生物学
计算成像技术的成像

背景情况:

光终身成像显微镜 (FLIM) 通过测量光衰变速率来在分子水平上可视化生物样本.
传统的FLIM面临着长时间的采集时间和广泛的扫描,特别是3D成像方面的挑战.
高分辨率的生命周期图对于理解分子相互作用和细胞环境至关重要.

研究的目的:

开发一种计算成像技术,克服传统FLIM系统的局限性.
为了能够更快,更有效地获取体积光寿命图像的数据.
为了减少高分辨率3D FLIM的扫描要求.

主要方法:

引入光场断层成像FLIM (LIFT-FLIM),一种新的计算成像方法.
使用具有高时间带宽的低维探测器,以有效获取数据.
使用线性单光子雪崩二极管阵列演示了LIFT-FLIM.

主要成果:

与传统的FLIM方法相比,LIFT-FLIM显著减少了扫描步骤.
实现了高数据效率的体积光寿命图像的获取.
展示了无与伦比的单光子检测灵敏度和扩展到光谱FLIM的功能.
应用LIFT-FLIM用于肺器官的高含量多重成像.

结论:

LIFT-FLIM为快速,高分辨率的3D光成像提供了强大的解决方案.
该技术增强了生物系统中的分子水平可视化.
LIFT-FLIM具有很大的潜力,可以促进基础和转化生物医学研究.

关键词:

在3D成像中使用3D成像.光终身成像显微镜显微镜光场成像光场成像

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