相关实验视频

Updated: Jun 12, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

在大鼠中通过UHPLC-Q-exactive轨道飞行物质谱仪识别daurisoline代谢物

Ruijun Cai¹, Jing Liu¹, Xuefang Wang¹

¹Department of Pharmacy, Shanghai General Hospital Jiuquan Hospital (The People's Hospital of Jiuquan), Jiuquan, GanSu 735000, China.

Journal of pharmaceutical and biomedical analysis

|September 25, 2024

概括

这项研究在老鼠中确定了63种达里索林代谢物,揭示了新的代谢途径. 了解这些代谢途径可以提高对达里索林的了解.

科学领域:

药理学药理学是指药理学的学科.
自然产品化学化学
代谢学代谢学代谢学

背景情况:

杜利索林是一种来自Menispermum dauricum的双基素类化合物,具有抗血小板,抗炎症,神经保护和抗瘤作用.
达里索林的体内代谢特征和代谢途径尚不完全理解.

研究的目的:

在Sprague-Dawley大鼠中全面调查daurisoline的体内代谢特征和途径.
识别和表征达里索林代谢物,阐明它们的代谢变化.

主要方法:

超高性能液体染色学与Q精确轨道轨道质谱学 (UHPLC-QE轨道轨道MS) 结合.
全扫描MS/dd-MS2,并行反应监测 (PRM),提取的离子染色学和诊断片离子被用于数据采集和分析.

主要成果:

在大鼠的血和组织中检测和表征了达里索林的63种代谢物.
确定了62种新型代谢物,以及可克劳林,表明了广泛的代谢转化.
关键的代谢反应包括脱,化,甲基化,硫化和葡萄糖化,阐明了裂变模式和组织分布.

结论:

这项研究提供了大瑞在老鼠体内的第一个全面的体内代谢概况.
鉴定的代谢物和途径加深了对达里索林代谢和药理作用的理解.
这项研究为进一步研究达里索林的治疗潜力奠定了重要的基础.

关键词:

达里索林 (Daurisoline) 是一种多利索林类物质.它的代谢物是代谢物.斯普拉格 - 达利老鼠在UHPLC-Q-Exactive轨道飞行器MSMS中使用.在这里,我们可以看到VIVO VIVO

更多相关视频

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

相关概念视频

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...