盐酸保护内皮细胞免受H2O2诱导的内皮功能障碍:通过EZH2调节炎症和自
Li Sun1, Xuefang Li2, Jie Zhang2
1Cardiovascular Research Center, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, China; Zhengzhou Sino-Crystal Diamond Co., Ltd., Zhengzhou, Henan 450001, China.
International immunopharmacology
|September 25, 2024
概括
沙利德化物 (SAL) 通过向增强体质同类物2 (EZH2) 来预防过氧化诱导的内皮功能障碍. 这种作用调节了自和炎症通路,为血管病变提供了潜在的治疗策略.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 内皮功能障碍是血管疾病的关键因素.
- 增强肠胃同源2 (EZH2) 的作用涉及到内皮功能障碍,是潜在的治疗点.
- 过氧化 (H2O2) 通常用于诱导氧化应激和模拟内皮功能障碍 in vitro.
研究的目的:
- 调查沙利德化物 (SAL) 对H2O2诱导的内皮功能障碍的保护机制.
- 阐明增强肠胃同源2 (EZH2) 在SAL治疗效果中的作用.
- 探索SAL对自和炎症信号通路的影响.
主要方法:
- 西方涂抹和RT-PCR用于量化蛋白质和基因表达.
- 划伤试验用于评估内皮细胞迁移.
- 分子对接以预测SAL和EZH2.2之间的相互作用.
- 在HUVEC中H2O2诱导的内皮功能障碍的体外模型.
主要成果:
- 沙利德化物 (SAL) 显著抑制了H2O2诱导的内皮细胞功能障碍.
- 盐酸治疗降低了关键炎症标志物 (NF-κB p65,NLRP3,TNF-α) 和与自相关的蛋白质 (Beclin1,LC3,P62) 的表达.
- 确定了SAL和EZH2之间的直接向关系,EZH2的敲击模仿了SAL的保护作用.
结论:
- 化 (SAL) 对H2O2诱导的内皮功能障碍产生保护作用.
- 通过调节自和炎症信号通路来调节SAL的功能,通过其与EZH2.2的相互作用来调节.
- 用SAL准EZH2为管理内皮功能障碍和血管病变提供了一个有希望的治疗途径.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:27Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
01:24Peroxisomes
11.2K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.2K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K