一种真空蛋白质MaSCPL1调解了蓝花葡萄黄的安东酸化修饰
Xiaoyun Cao1, Wenhui Hao1, Wanqi Pan1
1College of Landscape Architecture and Art, Northwest A & F University, Yangling, Shaanxi 712100, China; State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, Northwest A & F University, Yangling, Shaanxi 712100, China.
Plant science : an international journal of experimental plant biology
|September 25, 2024
概括
葡萄黄蓝色的颜色是由于安东素的修饰而产生的. 研究人员确定了MaSCPL1基因及其由MaMybA调节的基因,发现了花朵色彩的关键模块.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 葡萄黄表现出鲜的蓝色花朵,在装饰和工业上都受到重视.
- 血清素碳氧酸酶样乙转移酶 (SCPL-ATs) 基因家族对蓝色花的色素有着至关重要的作用.
- 了解SCPL-ATs在氨酸修饰中的作用是葡萄黄色彩的关键.
研究的目的:
- 为了识别SCPL-ATs参与葡萄黄的安托西亚宁修饰.
- 阐明控制MaSCPL1表达的调节机制及其在花颜色中的作用.
主要方法:
- 转录组分析以确定候选SCPL-AT基因.
- 基因表达概况和花再生试验用于验证基因功能.
- 酵母单杂交,EMSA和双露西法酶测定用于研究转录因子相互作用.
主要成果:
- 确定了马斯CPL1作为一个关键的基因,参与了氨酸修饰,其表达与花颜色相关.
- 沉默MaSCPL1降低了花中的总抗生素和DP3MG含量.
- MaMybA转录因子与MaSCPL1促进体结合并激活其表达,MaMybA-RNAi显著降低了MaSCPL1的表达.
结论:
- MaSCPL1基因与葡萄黄中的安托西亚尼酸性修饰有关.
- 调控模块MaMybA-MaSCPL1在葡萄黄花的色彩中起着至关重要的作用.
相关概念视频
Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
The Apoplast and Symplast
50.1K
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
50.1K
Protein Transport to the Outer Chloroplast Membrane
2.0K
Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
2.0K
Microtubule Associated Motor Proteins
7.7K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
7.7K
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Protein Transport to the Stroma
1.8K
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
1.8K


