ActO,一种积极的集群定位调节器,用于Streptomyces antibioticus ZS中actinomycins生物合成
Yingxin Liang1, Huaqiang Lu1, Jie Tang1
1School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education and Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
一种新型的调节剂,ActO,可以控制Streptomyces antibioticus ZS.中的阿克丁诺米辛生物合成. 增强ActO表达显着增加了D和V的生产,为改善抗生素产量提供了一个新的策略.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 动因菌素是具有显著治疗潜力的循环脂抗生素,特别是癌症治疗中的动因菌素D.
- 虽然已知actinomycin的特性和生物合成,但调节机制仍然不清楚.
- Streptomyces 抗生素 ZS 生产的是 D 和 V 类的 动胺素.
研究的目的:
- 描述S. antibioticus ZS.的actinomycin生物合成基因集群 (act集群) 中集群定位调节器ActO的功能.
- 调查ActO在调节动胺生物合成中的作用及其提高产量的潜力.
主要方法:
- 在S.antibioticus ZS.中,基因缺失和actO的过度表达.
- 定量实时PCR (RT-qPCR) 用于评估基因转录.
- 电泳运动转移试验 (EMSA) 用于评估蛋白质-DNA结合.
主要成果:
- 删除actO完全取消了阿克丁诺米辛的合成.
- 过度表达actO增加了D和V的产量,分别是4.4倍和2.6倍.
- ActO积极调节act集群内的所有基因的转录,尽管通过EMSA没有检测到直接促进体结合.
结论:
- ActO作为一种积极的调节剂,对氨酸生物合成至关重要,影响整个生物合成途径的转录.
- 过度表达actO代表了一种新且有效的策略,用于增加actinomycin产量.
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