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通过CBF通路,SlBTB19与SlWRKY2相互作用,通过CBF通路抑制番茄的寒冷耐受性

Jin Xu¹, Sidi Liu¹, Jiachen Hong¹

¹Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, People's Republic of China.

The Plant journal : for cell and molecular biology

|September 26, 2024

概括

番茄植物番茄植物

科学领域:

植物生物学植物生物学
分子生物学分子生物学
压力生理学压力生理学

背景情况:

广泛复杂的,电车轨道和bric-à-brac (BTB) 蛋白质对于植物发育和应激反应至关重要.
植物对寒冷应激耐受性的BTB蛋白的特定作用尚未完全理解.

研究的目的:

研究番茄 (Solanum lycopersicum) 中冷诱导的SLBTB19蛋白的功能及其在冷应激反应中的作用.
阐明SLBTB19调节寒冷耐受性的分子机制.

主要方法:

番茄中SLBTB19的过度表达和淘汰突变基因的产生.
蛋白质与蛋白质相互作用试验和细胞自由降解试验.
病毒诱导的基因沉默 (VIGS) 和促进体分析.

主要成果:

过度表达SLBTB19增加了对寒冷的敏感性,而SLBTB19的淘汰突变体表现出更强的寒冷耐受性.
SlBTB19与转录因子SlWRKY2.2相互作用并使其不稳定.
SlWRKY2在SLBTB19的下游作用,通过结合CBF1和CBF3促进体,通过CRT/DRE结合因子 (CBF) 途径积极调节寒冷耐受性.

结论:

一个SlBTB19-SlWRKY2模块负面调节西红中CBF依赖的耐寒路径.
SlBTB19 破坏了 SlWRKY2 的稳定,从而抑制了寒冷耐受性.
SlWRKY2是番茄寒冷耐受性的积极调节者,通过CBF通路起作用.

关键词:

BTBTB BTBTB 在线观看在CBF的CBF中,索兰 (Solanum lycopersicum) 是一种的植物.这就是WRKYY.寒冷耐受性耐寒性耐寒性转录规则转录规则转录规则

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