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在的设计Mycobacterium 结核病多表位粘合蛋白疫苗的粘合蛋白疫苗

Koobashnee Pillay¹, Thamsanqa E Chiliza², Sibusiso Senzani¹

¹Discipline of Medical Microbiology, School of Laboratory Medicine and Medical Sciences, College of Health Science, University of KwaZulu-Natal, South Africa.

|September 26, 2024

概括

针对Mycobacterium tuberculosis (Mtb) 粘附蛋白的新型多层细胞候选疫苗是使用免疫信息学设计的. 这些候选药物显示出高的预测免疫性,并具有针对结核病的亚单元疫苗开发潜力.

科学领域:

免疫学和疫苗学免疫学和疫苗学
计算生物学计算生物学
传染性疾病传染性疾病

背景情况:

结核菌菌 (Mtb) 粘附蛋白是子单元疫苗开发的潜在目标.
现有的疫苗策略需要加强,以有效预防结核病.

研究的目的:

使用免疫信息学设计和计算评估针对Mtb的新型多位候选疫苗.
识别并将Mtb粘合素的免疫原性表位物与稳定,非过敏性融合蛋白联系起来.

主要方法:

使用VaxiJen 2.0.0.对26MTB的粘附蛋白进行抗原潜力的in silico选,使用VaxiJen 2.0.0.
使用BepiPred2.0,ABCpred,SVMTriP和IEDB识别B细胞和T细胞表位.
使用C-ImmSim进行in silico免疫模拟和对人口覆盖率的分析.

主要成果:

选择的粘附蛋白 (tnHSP70,lpqH,MTP,PstS1) 用于构建两个三聚变蛋白:tnlpqH-tnPstS1-tnHSP70和tnMTP-tnPstS1-tnHSP70.
构造物被设计为稳定,水性,非过敏,并包含预测的B细胞和T细胞表位.
新型候选疫苗的高免疫性得分 (0.912-0.985) 被预测为新的疫苗候选.

结论:

设计的多表位聚变蛋白代表了有前途的,高度免疫的MTB的亚单元疫苗候选人.
这些in silico发现需要通过克隆,表达和净化进一步进行实验验证.

关键词:

粘附蛋白质是一种粘附蛋白质.免疫信息学是指免疫信息学.多种表位疫苗的疫苗.结核病菌菌菌的结核病菌的结核病菌.结核病是一种疾病.

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