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确定BCAT1具有致癌作用,并通过单细胞RNA-seq和实验在KIRC中促进EMT

Shiqing Li¹, Yinsheng Guo¹, Guanhua Zhu¹

¹Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Frontiers in oncology

|September 26, 2024

概括

分支链氨基酸转移酶1 (BCAT1) 通过增强表皮介质介质细胞过渡 (EMT) 来促进脏清细胞癌 (KIRC) 侵袭和转移. BCAT1显示出预后价值和作为KIRC的新生物标志物的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

脏清细胞癌 (KIRC) 是一种具有不良预后的侵袭性癌症亚型.
分支链氨基酸转移酶1 (BCAT1) 在KIRC病变发生中的作用在很大程度上是未知的.
了解BCAT1的功能对于开发新的KIRC治疗策略至关重要.

研究的目的:

研究KIRC中BCAT1的致癌作用和分子机制.
评估BCAT1作为KIRC患者潜在的预后生物标志物.
探索BCAT1表达与KIRC中的瘤微环境 (TME) 之间的关联.

主要方法:

对单细胞RNA-seq数据 (GSE159115) 和癌症基因组图谱 (TCGA) 数据库的分析.
生物信息分析包括基因组丰富分析 (GSEA),基因组变异分析 (GSVA),基因本体学 (GO) 和京都基因组百科全书 (KEGG).
实验验证使用西部斑点 (WB),免疫组织化学 (IHC) 染色,qRT-PCR和体外细胞检测 (过度表达/敲击) 的实验验证.

主要成果:

在KIRC组织中,BCAT1的表达很高,与预后不佳相关 (较短的OS,DFS,PFI,DSS).
BCAT1的表达与上皮介质转换 (EMT) 有关,并在体外促进KIRC细胞的入侵和迁移.
BCAT1促进体低甲基化可能会导致其在KIRC中的表达增加,从而影响瘤的进展和免疫透.

结论:

在KIRC中,BCAT1充当瘤基因,通过EMT促进入侵和转移.
BCAT1显示出显著的预后预测价值,并具有作为KIRC的新型诊断和治疗生物标志物的潜力.
向BCAT1可能为管理KIRC进展提供新的治疗途径.

关键词:

这是一个BCAT1BCAT1BCAT1BCAT1 在EMT中,EMT是EMT.生物标志物生物标志物细胞癌瘤是细胞癌.瘤免疫的微环境

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