双基酶 Ubp3/Usp10通过酸盐预算制约葡萄糖介导的线粒体抑制
Vineeth Vengayil1,2, Shreyas Niphadkar1,2, Swagata Adhikary1,2
1Institute for Stem Cell Science and Regenerative Medicine (DBT-inStem), Bangalore, India.
eLife
|September 26, 2024
概括
高葡萄糖通常会抑制线粒体呼吸. 一项研究发现,通过糖溶解减少无机酸盐 (Pi) 限制了线粒体的激活,而Ubp3控制了这一过程.
科学领域:
- 细胞代谢的细胞代谢.
- 线粒体功能 线粒体功能
- 生物化学 生化学
背景情况:
- 高葡萄糖的细胞往往抑制线粒体呼吸,被称为克拉布tree和Warburg效应.
- 在这些条件下线粒体激活的特定生化限制尚未完全理解.
研究的目的:
- 在高葡萄糖条件下确定调节线粒体抑制的因素.
- 阐明链接糖解和线粒体活动的生化机制.
主要方法:
- 在*Saccharomyces cerevisiae*中进行基因选,以确定参与线粒体抑制的基因.
- 在野生类型和突变细胞中对代谢流量,酶活性和无机酸盐 (Pi) 水平进行生物化学分析.
- 与Pi可用性和线粒体功能的相关发现的验证方法.
主要成果:
- 鉴定出duebiquitinase Ubp3 (Usp10) 对于高葡萄糖的线粒体抑制至关重要.
- Ubp3突变体表现出去压缩的线粒体活性,降低了糖解酶,以及重新连接的新陈代谢,有利于三糖的产生.
- 高糖解流消耗细胞内无机酸盐 (Pi),限制线粒体Pi的可用性,从而激活.
- 在Ubp3突变体中重新连接的新陈代谢恢复了Pi水平,导致线粒体脱压.
结论:
- 细胞内无机酸盐 (Pi) 预算受糖溶性流量的影响,是线粒体抑制的关键约束.
- Ubp3在调节这种依赖Pi的线粒体反应方面发挥着关键作用.
- 代谢灵活性和Pi的可用性是控制线粒体呼吸的核心,以应对营养的可用性.
关键词:
在GAPDH中使用.它们中的一种是S. cerevisiae.生物化学 生物化学癌症生物学 癌症生物学化学生物学 化学生物学葡萄糖溶解是什么无机酸盐是一种无机酸盐.代谢流量是指代谢的流量.线粒体中的线粒体.这是一种三糖.更多相关视频
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