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一种谷氨S转移酶调节了素生物合成,并增强了番茄的盐耐受性

Luqiao Yuan^1,2,3, Jiao Dang^1,2,3, Jiayue Zhang^1,2,3

¹College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.

Plant physiology

|September 26, 2024

概括

谷氨S转化酶U43 (SlGSTU43) 通过调节素生物合成,提高番茄的盐耐受性. 这种蛋白与SlCOMT2相互作用,并由SlMYB71和SlWRKY8控制,改善作物对盐度的抵抗力.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

盐应激会对作物生长和产量产生负面影响.
氨酸S转移酶 (GSTs) 在植物发育和应激反应中发挥作用.
在此之前,SlGSTU43在番茄盐耐受性方面的特定作用尚不清楚.

研究的目的:

在盐应激下研究番茄 (Solanum lycopersicum L.) 中的Glutathione S-transferase U43 (SlGSTU43) 的功能.
阐明SlGSTU43赋予盐分耐受性的分子机制.
为了确定控制SlgstU43表达的监管因素.

主要方法:

在野生类型和转基因番茄植物 (过度表达和淘汰突变) 上施加NaCl应激.
测量反应性氧物种清理和GST活动.
用于基因表达分析的RNA测序.
蛋白质与蛋白质相互作用的研究 (SlGSTU43和SlCOMT2).
对SlmYB71和SlWRKY8.8.进行促剂结合试验和基因沉默.

主要成果:

高NaCl度诱导了西红根中的Slgstu43表达.
过度表达 SlGSTU43 改善了反应性氧物种清理和盐耐受性,而淘汰突变体的耐受性降低.
SlGSTU43通过与SlCOMT2.2的相互作用来调节红素生物合成.
SlMYB71 和 SlWRKY8 在转录上激活 SlGSTU43 的表达.
沉默SlMYB71和SlWRKY8降低了GST活性,素含量和盐分耐受性.

结论:

SlGSTU43通过调节素生物合成,提高番茄的盐应激耐受性.
在盐应激下,SlGSTU43-SlCOMT2相互作用对调节素含量至关重要.
SlMYB71 和 SlWRKY8 作为 SlGSTU43 的上游调节剂,有助于盐分耐受性.
这项研究揭示了一种新的调节途径,用于植物的盐应激适应,涉及GST和素生物合成.

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