细胞内H2S水平的升高会增加大肠杆菌中铁的吸收
Shouta Nonoyama1, Shintaro Maeno2, Yasuhiro Gotoh3
1Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
mBio
|September 26, 2024
概括
大肠杆菌中的高细胞内硫化 (H2S) 水平通过增加铁的吸收来提高抗生素耐药性. 这种H2S-铁连接由YgaV转录因子介导,影响细菌的应激反应.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌生理学 细菌生理学
背景情况:
- 硫化 (H2S) 作为细菌的第二信使,影响氧化应激反应和抗生素耐药性.
- H2S和铁都是肠道细菌的关键信号分子,但它们的细胞内相互作用尚未得到充分理解.
- 以前的研究表明,细胞内H2S与抗生素耐药性之间存在联系.
研究的目的:
- 研究细胞内H2S过度积累对大肠杆菌的转录组的影响.
- 阐明H2S积累与大肠杆菌中铁吸收活动之间的关系.
- 扩大对细菌生理学中的H2S合成及其在抗生素耐药性中的作用的理解.
主要方法:
- 野生型大肠杆菌中mstA基因的过度表达诱导H2S过度积累.
- RNA测序 (RNA-seq) 分析以确定差异表达的基因.
- 测量细胞内铁含量和评估抗生素耐药性.
- 研究了YgaV转录因子在调节铁吸收基因中的作用.
主要成果:
- mstA过度表达导致细胞内H2S水平显著上升,抗生素耐药性增加.
- RNA-seq揭示了超过1000个基因的差异表达,高调基因参与铁的吸收.
- H2S过度积累增加了细胞内铁含量,由YgaV转录因子介导.
- YgaV对于H2S诱导的铁吸收基因上调是必不可少的.
结论:
- 细胞内H2S过度积累直接影响大肠杆菌中铁的可用性和吸收.
- YgaV转录因子通过细胞内H2S调节铁吸收基因.
- 这种H2S介导的铁调节对氧化应激反应和大肠杆菌抗生素耐药性至关重要.
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