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对一种新的微流体快速AST检测方法进行的多中心评估,用于检测格兰氏阴性血流感染

Benjamin Berinson¹, Emma Davies², Jessie Torpner²

¹Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Journal of clinical microbiology

|September 26, 2024

概括

快速MIC系统提供直接从血液培养物中快速抗菌感应性测试 (AST),在不到4小时内提供准确和可重复的结果. 这种创新方法增强了败血症患者的临床决策.

科学领域:

临床微生物学临床微生物学
诊断技术诊断技术诊断技术
传染病诊断传染病诊断传染病诊断

背景情况:

传统的抗微生物敏感性测试 (AST) 方法缓慢且劳动密集.
快速的AST对于指导治疗在时间关键的感染,如败血症至关重要.
现有的方法往往无法及时提供结果,延迟了适当的治疗.

研究的目的:

评估快速AST的QuickMIC系统的性能.
为了比较QuickMIC的速度,精度,准确性和可重复性与微稀释 (BMD).
评估该系统是否适合在临床环境中直接使用血液培养物.

主要方法:

使用微流体线性梯度的QuickMIC系统在四个实验室进行了测试.
使用尖端血培养 (n=411) 和临床血培养 (n=148) 评估了性能.
对12种抗生素的BMD进行了比较,使用10种定义的菌株评估了可再生性.

主要成果:

总体上,与 BMD 的基本一致 (95.6%) 和绝对一致 (96.0%) 是很高的.
错误率很低:非常严重 (1.0%),严重 (0.6%) 和轻微 (2.4%).
实验室间可重复性为98.9%,平均分析时间为3小时13分钟.

结论:

快速MIC提供直接从血液培养物中快速,准确和可重复的AST.
该系统在注射剂大小和延迟时间方面非常强大.
它的微流体设计确保了高的实验室间可重复性,使可靠的MIC值.

关键词:

诊断诊断诊断诊断诊断多中心研究多中心研究.快速的AST是一种快速的AST.这是一种血症.

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