改进了新型Calidifontibacillus erzurumensis LEV207的产量,使用一次变量方法
Palkar Omkar Prakash1, Keerthi Rayasam1, Vidyullatha Peddireddy2
1Department of Life Sciences, GITAM School of Science, GITAM (Deemed to Be University), Visakhapatnam, Andhra Pradesh, 530045, India.
概括
这项研究优化了海洋细菌的产量,实现了7倍的增长. 由此产生的外聚糖化物 (EPSLEV207) 由于其特性,显示出医疗和食品工业的潜力.
科学领域:
- 微生物学 微生物学
- 生物化学 生化学
- 生物技术是生物技术.
背景情况:
- 列万是一种外聚糖,在医疗和食品行业具有宝贵的应用.
- 微生物产生的Levon通常发生在低度.
- 优化对于提高工业可行性的利率至关重要.
研究的目的:
- 为了优化从一种新型海洋细菌,Calidifontibacillus erzurumensis LEV207.207. LEV207.207.207.207.207.207.207.207.207.207.207.207.207.207.207.207.207.207.207.
- 研究介质成分和条件对生长和levansucrase生产的影响.
- 描述产生的Levon (EPSLEV207) 并评估其功能性质.
主要方法:
- 一个变量一次优化介质成分 (糖,酵母提取物) 和条件 (pH,温度,MgSO4).
- 对levansucrase的酶活性测定.
- 使用塞瓦格试剂净化莱文.
- 使用NMR,XRD和FTIR进行结构和功能表征.
- 对抗氧化活性 (DPPH,ABTS) 和益生菌生长促进的评估.
主要成果:
- 在优化条件下 (pH 6.0,30°C,50%糖糖,1%酵母提取物,MgSO4),levansucrase活性显著增加.
- 莱万 (EPSLEV207) 的产量增加了七倍,最终产量达到65.58g/L.
- 证实EPSLEV207是一种由果糖组成的同聚糖,表现出抗氧化活性,促进益生菌生长,以及有利的水溶性/保持性.
结论:
- 优化的生产过程显著提高了C. erzurumensis LEV207.207的Levon产量.
- EPSLEV207具有理想的功能性质,表明其可用于工业应用的潜力.
- C. erzurumensis LEV207 是可持续和高效的生产的有希望的候选者.
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