诺基诺隆耐药性与Corynebacterium macginleyi在诺基诺隆耐药性决定区域的突变之间的关系
Nobuhiro Kato1, Masatoshi Haruta1, Rikki Arai1
1Department of Ophthalmology, Kurume University School of Medicine, Kurume, Japan.
Investigative ophthalmology & visual science
|September 26, 2024
概括
来自结膜的Corynebacterium macginleyi经常在决定类抗性的区域中表现出突变. 这些突变与对诺基诺的耐药性相关,影响眼科感染治疗.
科学领域:
- 眼科医生 眼科 眼科
- 微生物学 微生物学
- 遗传学 是一个遗传学.
背景情况:
- 结膜性细菌感染是眼科疾病的常见原因.
- 诺基诺是广泛使用的抗生素,用于治疗细菌性眼部感染.
- 了解抗生素耐药性机制对于有效治疗至关重要.
研究的目的:
- 分析结膜的细菌组成.
- 为了研究Corynebacterium macginleyi.yi.中的基诺抗性.
- 确定诺隆耐药性决定区域 (QRDR) 突变与C. macginleyi. 的诺耐药性之间的相关性.
主要方法:
- 从接受眼科手术或怀疑患有角膜结膜炎的患者收集结膜拭子.
- 鉴定了细菌分离物,重点关注的是Corynebacterium macginleyi.yi.
- 对于C. macginleyi分离物来说,基诺的最低抑制度 (MIC) 已确定.
- 在C. macginleyi中测序了gyrA基因的QRDR,以确定突变.
主要成果:
- 在结膜培养中,Corynebacterium macginleyi是最常见的生物体 (34.8%).
- 91.8%的C. macginleyi分离物在QRDR中表现出氨基酸突变.
- 在Ser-87和Asp-91的突变是最常见的 (95.5%).
- 具有Ser-87和Asp-91双重突变的分离物对多代化类具有耐药性.
结论:
- 结膜C. macginleyi与QRDR突变的分离物对化诺具有耐药性.
- QRDR突变是C. macginleyi. 诺基诺隆耐药性的重要因素.
- 这一发现对治疗这种细菌引起的眼部感染有意义.
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