细菌中的5'-NAD RNA帽的随机性质和生理影响
Jana Wiedermannová1,2, Ravishankar Babu1, Yulia Yuzenkova1
1Centre for Bacterial Cell Biology, Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4AX, UK.
Nucleic acids research
|September 26, 2024
概括
在大肠杆菌中,当ATP水平较低时,RNA5'-modification与尼古丁胺胺氨酸二核酸 (NAD) 发生. 这种NADylation影响RNA的稳定性和降解,影响基因表达.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 遗传学 遗传学 是一个
背景情况:
- 在细菌,真核生物和病毒中观察到RNA 5缩写{'}与尼古丁胺胺二核酸 (NAD+/NADH) 的修饰.
- RNA聚合酶 (RNAPs) 在RNA合成启动过程中结合了5个缩写NAD.
- 控制活体RNANADylation及其对细菌基因表达的影响的关键因素和生理条件在很大程度上仍未被描述.
研究的目的:
- 阐明在大肠杆菌中促进大量RNANADylation的条件.
- 研究5个缩写的NAD整合对细菌中的基因表达和RNA命运的功能后果.
主要方法:
- 研究了ATP度和RNAP活性位点相互作用对RNANADylation的影响.
- 评估了DNA超级卷对RNANADylation的影响.
- 研究了RNA NADylation对RNase E和RNAI/RNAII基配对的转录后处理的影响.
- 研究了NADylated mRNA的翻译效率及其易受NudC.de-capping的影响.
- 分析了ppGpp在调节NudC脱活动和NADyllated RNA水平中的作用.
主要成果:
- 大肠杆菌中的RNA NADylation是由低ATP度和弱化的ATP-RNAP接触增强的.
- DNA超卷也可能影响RNA NADylation水平.
- 纳基基化不会阻碍RNase E处理或RNAI/RNAII基配对.
- 纳迪化mRNA被70S核糖体高效地翻译,而5个缩写{'}NAD盖被暴露为NudC介导的脱盖.
- ppGpp 抑制了 NudC 脱,将 NAD 基化 RNA 水平与细菌生长阶段联系起来.
结论:
- RNA NADylation是由细胞ATP水平和RNAP相互作用调节的.
- 由于NudC脱,NADylated mRNA可能在翻译后迅速降解.
- 增长阶段依赖的NAD基化RNA的积累受到ppGpp介导的NudC活性抑制的影响.
相关概念视频
00:43Bacterial RNA Polymerase
29.4K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.4K
01:23Types of RNA
63.4K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.4K
02:43Nucleic Acids
43.9K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.9K
02:51mRNA Stability and Gene Expression
5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.6K
01:25Nucleic Acid Structure
6.0K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
DNA Structure
DNA...
6.0K
02:53Ribosomal RNA Synthesis
13.1K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.1K