相关实验视频
Updated: Jun 12, 2025

06:05
Additive Manufacturing-Enabled Low-Cost Particle Detector
Published on: March 24, 2023
1.2K
功率函数值在充电式气溶探测器的线性和普遍性中的作用:来自验证模型的理论阐释
Troy T Handlovic1,2, Daipayan Roy1, Wesley W Barnhart1
1Amgen Research, One Amgen Center Drive, Thousand Oaks, California 91320, United States.
Analytical chemistry
|September 26, 2024
概括
这项研究引入了一个模型来预测充电的气溶探测器 (CAD) 信号,优化药物发现中的量化. 该方法通过精确选择功率函数值来进行液态染色学-CAD分析来提高准确性.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 药物发现 药物发现 药物发现
背景情况:
- 高通量药物发现需要准确的分析量化,而无需对分子进行特定校准.
- 充电式气溶探测器 (CAD) 在液态染色学 (LC) 中为非挥发性和非染色体化合物提供通用检测.
- CAD固有的非线性质量响应挑战了传统的量化,需要优化"功率函数值" (p).
研究的目的:
- 根据p=1.1时收集的数据,开发一种理论关系,用于预测LC-CAD染色图在任何"p"值时的预测.
- 使用多样化的数据集验证预测模型,并提供用于优化"p"值的自动化代码.
- 为了证明如何优化"p"值可以最大限度地减少或消除低水平的杂质,并提高定量准确性.
主要方法:
- 开发一个理论模型,以预测不同"p"值的峰值面积,宽度和高度.
- 模型的验证使用LC-CAD分析的1440次测量.
- 实现和测试开放式自动化代码,用于预测最佳的"p"值.
主要成果:
- 预测模型与实验结果有很好的一致性,预测区域的平均误差低于2%.
- 优化"功率函数值"被证明会不成比例地影响不同高度的峰值.
- 提高"功率函数值"有效地减少和消除了低水平的杂质.
结论:
- 开发的理论关系和自动化代码为优化LC-CAD方法提供了一个易于实现的工具.
- 这种方法显著提高了方法开发的速度,并提高了药物发现中的定量工作流程的准确性.
- 该模型有助于选择最佳的"p"值,提高了对非挥发性分析物的量化可靠性.
相关概念视频
Atomic Emission Spectroscopy: Overview
1.6K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
1.6K
Gas Chromatography: Types of Detectors-II
343
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
343
High-Performance Liquid Chromatography: Types of Detectors
506
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
506
Atomic Emission Spectroscopy: Lab
150
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
150
Mass Analyzers: Common Types
579
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
579
Gas Chromatography: Overview of Detectors
451
Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
451

