相关实验视频

Updated: Aug 7, 2026

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

拉波尼特改性甲基基酸水凝,可控制释放血管内皮生长因子a,用于骨再生

Baoguo Mi¹, Jitao Zhang¹, Hailan Meng¹

¹Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710054, China.

Biochemical and biophysical research communications

|September 26, 2024

概括

这项研究介绍了一种可注射的水凝 (G@Lap/VEGF) 用于骨修复,它结合了Laponite和血管内皮生长因子. 这种新型生物材料在动物模型中有效促进骨再生和新血管化.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
组织工程是组织工程.

背景情况:

骨缺陷重建面临的挑战是当前的治疗方法,如骨移植.
可注射生物仿真水凝通过输送细胞和生长因子,为骨再生提供了最少的侵入性方法.
新的策略对于推进骨修复解决方案至关重要.

研究的目的:

设计和制造一个可注射的纳米复合物水凝 (G@Lap/VEGF) 与拉波尼特 (Lap) 和血管内皮生长因子 (VEGF) 共同装载.
评估水凝在促进骨再生和新血管化的潜力.

主要方法:

凝MA,拉波尼特 (Lap) 和血管内皮生长因子 (VEGF) 的物理混合,以创建G@Lap/VEGF水凝.
在体外评估Laponite对水凝注射能力和VEGF释放动力学的影响.
评估拉波尼特对介质干细胞 (MSC) 骨质分化和矩阵矿物化的影响.
评估VEGF在招募内皮原生细胞 (EPC) 和诱导新血管化的作用.
在Sprague-Dawley (SD) 鼠中临界大小的骨缺陷的体内测试,使用组织学和微型计算机断层扫描 (micro-CT) 分析.

主要成果:

该G@Lap/VEGF水凝证明了最佳的注射性和持续的VEGF释放.
拉波尼特增强MSC骨质生化生物标志物表达和矩阵矿化.
VEGF有效地招募了EPC,并促进了新血管化.
在体内研究显示,在用G@Lap/VEGF.治疗的骨缺陷中,有显著的骨修复.

结论:

可注射的G@Lap/VEGF纳米复合物水凝是临床骨修复的有希望的生物材料.
这种简化的水凝系统为骨再生应用提供了方便的方法.
该研究有助于开发用于组织工程的先进注射生物仿真水凝.

关键词:

骨再生骨的再生有控制释放的释放.凝甲基烯基基凝拉波尼特人 (Laponite) 是一种拉波尼特人.在VEGF-A的基础上.

更多相关视频

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Published on: May 19, 2023

相关概念视频

Bone Remodeling

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Bone Remodeling and Repair

Bone Remodeling and Repair

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...