转录组分析显示,细胞循环调节中的缺陷有助于植入前胚胎停止
Xin Li1, Yang Zou2, Baobao Geng3
1Reproductive Medicine Center, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi Province, China; JXHC Key Laboratory of Fertility Preservation, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi Province, China.
Genomics
|September 26, 2024
概括
植入前胚胎停止 (PREMBA) 是IVF失败的主要原因之一. 我们的研究确定了细胞周期基因失调,包括CCNB2和CDC25A,是胚胎发育失败的关键因素.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 植入前胚胎停止 (PREMBA) 显著影响辅助生殖技术的成功.
- 尚不完全了解PREMBA的分子基础,这阻碍了有效的治疗策略.
研究的目的:
- 为了阐明驱动PREMBA的分子机制.
- 为了确定潜在的生物标志物和治疗目标的复发性植入失败.
主要方法:
- 单个胚胎转录组测序PREMBA和正常的人类胚胎.
- 对比转录基因分析和加权基因共同表达网络分析 (WGCNA).
- 使用蛋白质-蛋白质相互作用网络和RT-qPCR识别和验证枢纽基因;在小鼠囊细胞和HEK293T细胞中进行功能验证.
主要成果:
- 细胞循环阶段过渡的调节失调被确定为PREMBA的潜在致病因子.
- 确定了包括CCNB2,BUB1B,CDC25A,CCNB3和PLK3在内的关键枢纽基因.
- 在小鼠生殖器中抑制CCNB2会影响胚胎发育,而抑制CDC25A会影响HEK293T细胞的细胞增殖和细胞亡.
结论:
- 细胞周期基因失调,特别是涉及已识别的枢纽基因,在植入前胚胎停产中起着关键作用.
- 这些发现为预测胚胎发育结果提供了潜在的生物标志物.
- 已识别的枢纽基因代表了改善试管婴儿/ICSI成功率的有希望的治疗点.
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