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由单细胞RNA-seqq揭示的牙组织衍生的MSC中的异质性

C Behm¹, O Miłek¹, K Schwarz¹

¹Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria.

Journal of dental research

|September 27, 2024

概括

来自牙组织的间酶体 stromal 细胞 (MSC) 在组织和捐赠者之间显示出有限的变异性. 在单细胞水平上了解这些差异是有效的基于细胞的疗法的关键.

科学领域:

细胞生物学细胞生物学
再生医学是一种再生医学.
基因组学就是基因组学.

背景情况:

介酶体 stromal 细胞 (MSCs) 对治疗退行性和炎症性疾病充满希望.
由于组织和供体变异的MSC异质性限制了治疗用途.
单细胞转录组学对于理解MSC多样性至关重要.

研究的目的:

调查从牙周带 (PDL) 和牙上产生的MSC的组织特异性和供体特异性转录组变异性.
在牙组织中识别不同的MSC亚群.
评估组织来源和个体捐赠者对MSC特征的影响.

主要方法:

从PDL和5名健康个体的牙中分离和体外培养MSCs.
3,844个转录组的单细胞mRNA测序.
生物信息分析包括聚类,基因/通路丰富和蛋白质-蛋白质相互作用 (PPI) 网络分析.

主要成果:

识别PDL和牙特异性和组织跨越的MSC亚群.
组织特异性MSC亚群之间细胞过程的有限差异.
在MSC转录组中观察到显著但受限的捐赠者对捐赠者的变异性.

结论:

牙组织衍生的MSCs表现出特定于组织和供体的转录组变异,主要影响特定的细胞过程.
在牙组织中存在不同的MSC亚群,突出显示细胞间差异.
在移植之前,建议在单细胞水平上控制MSC特性,以治疗应用.

关键词:

基因表达分析基因表达分析的的的介质细胞层细胞.牙周关节带牙周关节带一个单细胞分析.翻译学翻译学翻译学翻译学

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...