在IL-1β诱导的骨关节炎中,CircSEC24A的调节作用和机制
Yuanrui Wang1, Xiaochao Chen1, Yongfeng Chen1
1Department of Orthopaedics, Xijing Hospital, The Air Force Military Medical University, Xi'an, Shaanxi, China.
Archives of physiology and biochemistry
|September 27, 2024
概括
像circSEC24A这样的循环RNA (circRNAs) 参与了骨关节炎 (OA) 的发病. 针对circSEC24A/miR-515-5p/NAMPT轴可能为OA提供一种新的治疗策略.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 整形外科 整形外科 整形外科
背景情况:
- 骨关节炎 (OA) 是一种退行性关节疾病,患病率越来越高.
- 循环RNAs (circRNAs) 正在成为OA病变发生的关键参与者.
- 在OA中,Hsa_circ_0128006 (circSEC24A) 受到升级监管,但其作用尚不清楚.
研究的目的:
- 研究circSEC24A在骨关节炎中的生物学作用和机制.
- 阐明涉及OA中circSEC24A,miR-515-5p和NAMPT的调节途径.
主要方法:
- 在OA软骨和IL-1β治疗细胞中分析circSEC24A,NAMPT和miR-515-5p的表达.
- 在体外实验中评估circSEC24A敲击对细胞过程的影响.
- 研究circSEC24A的分子海绵机制,用于miR-515-5p.
主要成果:
- 在OA组织和细胞中,circSEC24A和NAMPT被上调,而miR-515-5p被下调.
- circSEC24A敲击逆转了IL-1β诱导的状细胞损伤,包括降低活力,增加氧化应激,亡,ECM降解和炎症.
- circSEC24A 作为 miR-515-5p 的分子海绵,调节 NAMPT 的表达.
结论:
- 循环SEC24A通过海绵化miR-515-5p和调节NAMPT来加剧骨关节炎的进展.
- 准circSEC24A/miR-515-5p/NAMPT轴为骨关节炎治疗提供了一个潜在的治疗途径.
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