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数字空间分析识别了与HPV和p16状态相关的口腔瘤内与免疫瘤相关的基因表达的独特模式

Jill M Brooks¹, Yuanning Zheng², Kelly Hunter³

¹Institute of Head and Neck Studies and Education, Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, United Kingdom.

Frontiers in oncology

|September 27, 2024

概括

人类乳头瘤病毒 (HPV) 媒介的口腔瘤 (OPC) 显示出更好的预后. 双重p16和HPV测试对于OPC患者的准确诊断和治疗决策至关重要.

科学领域:

在瘤学瘤学.
病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

口腔癌 (OPC) 的发病率正在上升,主要是由于人类乳头瘤病毒 (HPV) 感染.
通过HPV介导的OPC的预后比HPV阴性OPC更好,这引起了人们对治疗减强的兴趣.
目前针对HPV状况的p16免疫组织化学测试可能会错误分类病例,影响临床管理和预后.

研究的目的:

探索p16/HPV不一致的OPC病例的潜在生物学.
为了比较不同OPC亚组 (p16+/HPV+, p16+/HPV-, p16-/HPV-) 的免疫瘤基因表达模式.
评估双重p16和HPV测试对指导临床决策的有用性.

主要方法:

使用GeoMx数字空间分析来分析基因表达.
73个与免疫瘤学相关的基因在瘤和脑膜瘤区中得到量化.
分析包括来自p16+/HPV+,p16+/HPV-和p16-/HPV-亚组的12个甲固定,嵌的OPC组织.

主要成果:

与p16+/HPV+相比,p16+/HPV-和p16-/HPV-瘤中的氨酸 (KRT) 和HIF1A被上调调节.
与增殖相关的基因 (CCND1,AKT1,CD44) 在p16-/HPV-瘤中较高.
免疫反应基因 (CXCL9,CXCL10,ITGB2,PSMB10,CD74,HLA-DRB,B2M) 在p16+/HPV+瘤中较高.

结论:

不一致的p16+/HPV-OPCs表现出更类似于p16-/HPV-OPCs的基因表达模式.
这些发现与已知的预后差异一致:p16+/HPV+ >> p16+/HPV- > p16-/HPV-.
双重p16和HPV测试对于准确的预后评估和OPC的临床决策至关重要.

关键词:

数字空间配置文件数字空间配置文件人类乳头瘤病毒 (HPV)双重的p16/HPV测试可以进行.这是口腔喉癌.p16 p16 在这个问题上.处理减强处理的强化.瘤微环境是一个微环境.

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