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牙髓干细胞的特定细胞子集群通过ROS途径对不同的病原体作出反应

Tiansong Xu^1,2, Yangjia Liu¹, Wen Zhang^3,4

¹Central Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.

Frontiers in cellular and infection microbiology

|September 27, 2024

概括

牙髓干细胞 (DPSCs) 显示出一个独特的子集群,DPSC.7,它特别对真菌感染作出反应. 这种反应涉及激活免疫路径,建议治疗口腔真菌病原体的新策略.

科学领域:

口腔微生物学口腔微生物学
干细胞生物学干细胞生物学
免疫学免疫学免疫学

背景情况:

口腔病原体如 *Candida albicans* 和 *Streptococcus mutans* 可以感染人体器官,包括口腔.
牙纸干细胞 (DPSC) 对于牙组织再生和免疫反应至关重要.
了解DPSC对特定病原体的反应对于开发向疗法至关重要.

研究的目的:

研究DPSCs对口腔真菌和细菌病原体的反应中的细胞和分子特征.
为了确定特定的DPSC亚群,这些亚群对*Candida albicans*感染表现出独特的反应.
阐明涉及DPSC介导的免疫反应对口腔病原体的分子途径.

主要方法:

从受影响的人类第三牙中分离和培养DPSCs.
用 *Candida albicans* (C.a), *Streptococcus mutans* (S.m) 或细胞介质 (Ctrl) 刺激DPSCs.
单细胞mRNA测序分析以表征DPSC响应在转录基因水平.

主要成果:

鉴定出一个不同的DPSC子集群,DPSC.7,在C.a刺激组中流行率显著更高.
DPSC.7细胞显示出与活性氧物种 (ROS) 反应和特征聚合相关的独特基因表达模式.
在C.a刺激后,在DPSC.7细胞中观察到MAPK/ERK1/2和NF-κB通路的激活,抑制*DUSP1/5/6*和诱导具有高细胞因子水平 (IL-6,CCL2) 的免疫相关通路.

结论:

单细胞RNA测序揭示了一个特定的DPSC子集群 (DPSC.7),它对口腔病原体产生独特的免疫反应.
DPSC.7反应涉及ROS,MAPK和NF-κB通路,导致免疫激活.
这些发现为管理口腔真菌感染的新型治疗策略提供了洞察力.

关键词:

在Candida albicans中使用.这就是FOSOS.在MAPK/ERK1/2 牙髓干细胞是牙髓的干细胞.免疫反应的免疫反应.

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