欧戈氨酸补充剂对介质能力和猪卵细胞发育的影响
Megumi Nagahara1,2, Zhao Namula1,3, Qingyi Lin1,2
1Bio-Innovation Research Center, Tokushima University, Tokushima, Japan.
Veterinary world
|September 27, 2024
概括
埃尔戈氨酸 (EGT) 补充剂通过减少DNA碎片化来改善猪卵细胞的成熟和质量. 这有助于胚胎发育,尽管EGT的抗氧化机制需要进一步研究.
科学领域:
- 生殖生物学 生殖生物学
- 细胞抗氧化剂防御的细胞抗氧化剂防御
- 胚胎发育 胚胎发育
背景情况:
- 埃尔戈西欧内因 (EGT) 在猪细胞培养中的抗氧化作用尚不清楚.
- 猪卵细胞成熟和发育能力对于高效的辅助生殖技术至关重要.
研究的目的:
- 调查欧戈氨酸 (EGT) 补充对猪卵细胞中介性成熟和发育潜力的影响体外.
- 评估不同EGT度对卵细胞质量和随后的胚胎细胞形成的影响.
主要方法:
- 猪卵细胞在体外成熟,EGT的度不同 (0,5,10,25,50,100微米).
- 评估了精子进化,受精率和胚胎细胞形成.
- 卵细胞和胚胎囊中的DNA碎片化水平被量化以评估质量.
主要成果:
- 与对照组相比,EGT补充剂显著增加了基因相II卵细胞的比例.
- 培养≥10μMEGT的卵细胞显示DNA碎片化减少.
- 由10μMEGT成熟的卵细胞衍生出的胚胎囊显示出最高的发育率,尽管总细胞数量和DNA碎片化指数不受影响.
结论:
- 在体外成熟期间补充EGT可以提高猪卵细胞的成熟,质量和胚胎发育,主要是通过减少DNA碎片化.
- 通过EGT减少猪卵细胞中DNA碎片化的精确抗氧化机制仍需要阐明.
- 需要进一步的研究,以充分了解EGT在生殖环境中影响的抗氧化途径.
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