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在Metarhizium brunneum中调节草类生物合成的基因

Samantha J Fabian¹, Chey R Steen¹, F Heath Damron²

¹Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, USA.

Applied and environmental microbiology

|September 27, 2024

概括

一个新发现的基因,easR,对于菌的藻类合成至关重要. 它的缺失阻止了藻类化物的产生,并影响了昆虫中的真菌毒性,这表明它具有关键的调节作用.

科学领域:

菌类学菌类学是指菌类学.
生物化学生物化学
遗传学遗传学是一个

背景情况:

菌根中的基因集群对于生产具有农业相关性和制药价值的化合物至关重要.
虽然生物合成基因已知,但控制藻类化物生产的调节机制在很大程度上仍未被描述.

研究的目的:

为了研究一种新型基因easR的功能,该基因在Metarhizium brunneum.brunneum中与eas集群相邻.
确定easR在调节藻类类生物合成中的作用及其对真菌毒性的影响.

主要方法:

在Metarhizium brunneum中使用CRISPR-Cas9基因淘汰的easR基因的表征.
在感染野生型和easR淘汰菌株的Galleria mellonella幼虫中积聚藻类的分析.
使用RNAseq和RT-PCR进行基因表达分析,以评估eas集群基因转录.

主要成果:

easR淘汰菌株的M.brunneum显示完全没有可检测的ergot类化合物.
与野生类型相比,easR淘汰菌株中,eas集群基因的转录显著减少或被废除.
感染easR淘汰菌株的幼虫的生存率显著增加,这表明真菌毒性减少.

结论:

easR基因产物对于藻类化物积累至关重要,并调节藻类化物生物合成基因的表达.
EasR在M.brunneum对昆虫的毒性中发挥着作用,间接突出了藻类的作用.
easR的同类物存在于其他生长藻类的真菌中,这表明一种保存的调节机制.

关键词:

克拉维西皮塔家族 (Clavicipitaceae) 是一个甲二是一种.生物合成基因集群生物合成基因集群埃尔戈特类化合物埃尔戈特类化合物 lysergic 酸的氨基酸胺.

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