相关实验视频
Updated: Jul 13, 2026

20:59
Large Insert Environmental Genomic Library Production
Published on: September 23, 2009
15.9K
通过自动化方式获得正确的克隆:一种替代复杂的殖民地挑选机器人的替代方案
Lorena Hägele1, Brian F Pfleger2, Ralf Takors1
1Institute of Biochemical Engineering, University of Stuttgart, 70569 Stuttgart, Germany.
Bioengineering (Basel, Switzerland)
|September 27, 2024
概括
本研究介绍了一种自动化液体克隆选择 (ALCS) 方法,用于应变工程. ALCS为生物造厂提供了低技术,高度选择性的替代品,改善了设计-建造-测试-学习循环.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物基础设施自动化自动化
背景情况:
- 设计-建造-测试-学习 (DBTL) 周期对于应变工程至关重要.
- 自动化生物基础设施简化了DBTL,但克隆选择仍然是一个瓶.
- 半自动化设施需要负担得起,有效的克隆选择解决方案.
研究的目的:
- 开发一种低技术,高度选择性的克隆选择方法,用于生物发掘厂.
- 解决半自动化设施的克隆选择中的瓶问题.
- 在学术和工业环境中实现高效的应变工程.
主要方法:
- 开发了自动化液体克隆选择 (ALCS) 方法.
- 在基于模型的克隆选择设置中实施ALCS.
- 与多种细菌底盘生物体进行ALCS测试,包括大肠杆菌, Pseudomonas putida和Corynebacterium glutamicum.
主要成果:
- 在五代内,对正确转变的细胞实现了98 ± 0.2%的选择性.
- 对初始细胞数量的变化表现出强度.
- 成功地将ALCS应用于各种细菌底盘生物体,使其能够立即用于下游应用.
结论:
- ALCS提供了一种简单的"低技术"解决方案,用于在生物造厂中进行克隆选择.
- 该方法提高了DBTL循环的效率,而不需要额外的专用设备.
- ALCS是一种适用于各种底盘生物的多功能和强大的工具,用于应变工程.
更多相关视频
10:51High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Published on: December 5, 2016
9.8K
09:13Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
Published on: January 17, 2019
7.3K
相关概念视频
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...