相关实验视频

Updated: Jun 12, 2025

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

在非小细胞肺癌中循环外体miRNA配置文件

Abeer A I Hassanin^1,2, Kenneth S Ramos¹

¹Center for Genomic and Precision Medicine, Texas A&M Institute of Biosciences and Technology, Texas Medical Center, Houston, TX 77030, USA.

|September 27, 2024

概括

循环的外体微RNA在肺癌患者中显示出不同的特征. 这些特定的微RNAs (miRNAs) 可能在非小细胞肺癌 (NSCLC) 的发展和进展中发挥作用.

科学领域:

生物化学生化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

微RNAs (miRNAs) 越来越多地被认可为它们在癌症中的作用,它们可以作为瘤基因或瘤抑制剂.
外基因组促进细胞间通信,它们内的miRNA可能会调解这些信号.

研究的目的:

在肺癌患者中调查循环外体miRNA的概况.
确定与状细胞肺癌 (SQCLC) 和肺腺癌 (LUAD) 相关的特定外体miRNA.

主要方法:

在健康对照组,SQCLC患者和LUAD患者中分析了25个循环外体微RNA.
选择八种高度丰富的miRNAs进行进一步的研究,根据它们在研究队列中的流行情况.

主要成果:

鉴定了八种特定的miRNAs (miR-21-5p,miR-126-3p,miR-210-3p,miR-221-3p,Let-7b-5p,miR-146a-5p,miR-222-3p和miR-9-5p) 的发现.
这些miRNA被检测到非小细胞肺癌 (NSCLC) 患者在所有阶段和性别.
外体miRNA水平与瘤临床病理学特征相关.

结论:

循环的外体miRNAs,特别是已识别的八个,是NSCLC的潜在生物标志物.
这些miRNAs可能有助于NSCLC中的致癌活性和细胞间信号传递.

关键词:

在NSCLCLC中,我们可以看到NSCLCLC.诊断性表现的诊断性表现是什么外基因组是外基因组的组成部分.基因本体学基因本体学这就是miRNAs.

更多相关视频

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...