基于氨酸和马莱胺的生物活性水凝功能化德克斯用于组织工程应用
Lin Zhong1, Alma Tamunonengiofori Banigo1, Bram Zoetebier1
1Department of Developmental BioEngineering, Faculty of Science and Technology, TechMed Centre, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands.
Gels (Basel, Switzerland)
|September 27, 2024
概括
研究人员使用酶催化交联和生物功能化开发了用于组织工程的生物活性水凝. 这些基于德克斯的水凝支持细胞活力和分化,为组织再生提供了多功能平台.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 生物化学 生物化学
背景情况:
- 在组织工程中,水凝对于模仿细胞外基质 (ECM) 和支持细胞功能至关重要.
- 基于德克斯的水凝虽然在结构上具有优势,但缺乏固有的生物活性,阻碍了它们的应用.
- 开发生物活性水凝对于推进组织工程战略至关重要.
研究的目的:
- 通过使得德克斯与胺和马莱胺组功能化,制造生物活性水凝.
- 为了实现可调节的水凝特性,酶催化交联.
- 为了研究水凝硬度和RGD结合对人类介质干细胞 (hMSC) 差异化的影响.
主要方法:
- 顺序的醇-马莱胺生物功能化德克斯.
- 用过氧化酶 (HRP) 和过氧化 (H2O2) 进行酶催化交联.
- 加入 arginylglycylaspartic acid (RGD) 来增强其生物活性.
- 封装hMSCs以评估细胞活力和在不同的水凝条件下的分化.
主要成果:
- 可调节的水凝凝时间和刚度是通过调整H2O2度来实现的.
- 在水凝中保持了高hMSC活力 (在第一天超过88%,在第14天达到60-81%).
- 水凝促进了hMSCs的基分化,油红O染色证实了这一点.
- 水凝的刚度和RGD度影响了hMSC差异化结果.
结论:
- 德克斯塔-马尔水凝提供了一个生物相容的支架,支持细胞活力并促进分化.
- 开发的水凝为组织工程应用提供了一个多功能平台.
- 这一策略增强了用于再生医学的基于德克斯的水凝的生物功能.
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