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评估基于基因组的平均核酸身份计算,用于识别12种酵母菌种

Claudia Cortimiglia¹, Javier Alonso-Del-Real², Mireya Viviana Belloso Daza¹

¹Department for Sustainable Food Process (DISTAS), Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy.

Journal of fungi (Basel, Switzerland)

|September 27, 2024

概括

一个用于细菌识别的工具FastANI通过计算平均核酸标识 (ANI) 来有效地分类酵母菌株. 这种基因组方法为酵母物种划分提供了一种可靠和有区别的方法,即使是复杂的杂交病例.

科学领域:

菌类学菌类学是指菌类学.
基因组学就是基因组学.
纳税学是一种分类学.

背景情况:

酵母菌株的分类具有挑战性,需要不同的表型和分子方法.
基因组分析对于准确的分类学划分越来越重要.
现有的方法可能缺乏精确的酵母物种分配所需的区分能力.

研究的目的:

为了评估使用FastANI进行酵母物种识别的平均核酸标识 (ANI).
将FastANI的性能与已知的分子方法 (如D1/D2 LSU rRNA和MAOG) 相比较.
评估FastANI在区分密切相关的酵母物种和杂交菌株方面的实用性.

主要方法:

使用FastANI对来自12个属的644个酵母组合进行了分析.
与in silico D1/D2 LSU rRNA基因序列和多重对齐的正确基因 (MAOG) 的比较.
适用于混合 *Saccharomyces* 种类的数据集.

主要成果:

在FastANI和MAOG结果之间观察到很高的一致性.
与D1/D2 LSU rRNA和MAOG相比,FastANI在基因组序列上表现出优异的歧视.
快速ANI有效地划分了具有明确界限的物种 (94-96%的ANI截止值) 并确定了杂交菌株.

结论:

FastANI是一种可靠和有区别的工具,用于将酵母菌株分配给已知的物种.
该方法即使在涉及杂交事件的复杂案例中也是有效的.
应将FastANI整合到全面的酵母物种划分策略中,以便使用全基因组序列快速识别.

关键词:

一个快速的FASTANI.多个位置的特征表征.分类基因组学是一门学科.整个基因组的测序.酵母物种的划分酵母物种的划分

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