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使用2DGC-GC-MS平台区分复发性和渐进性多发性硬化症的非目标代谢学方法的实用性

Indrani Datta^1,2, Insha Zahoor³, Nasar Ata³

¹Department of Public Health Sciences, Henry Ford Health, Detroit, MI 48202, USA.

|September 27, 2024

概括

这项研究在多发性硬化症 (MS) 患者中确定了独特的血清代谢物概况,将复发性复发性硬化症 (RRMS) 和初级进展性硬化症 (PPMS) 与健康受试者 (HS) 区分开来. 这些代谢指纹可以帮助诊断多发性硬化和其他进展性自身免疫性疾病.

科学领域:

神经免疫学神经免疫学
代谢学代谢学代谢学
生物化学生化学

背景情况:

多发性硬化症 (MS) 是一种常见的神经退行性疾病,影响年轻人,其特点是具有明显的疾病过程,如复发性复发性MS (RRMS) 和初级渐进性MS (PPMS).
基于血清代谢物概况,在区分这些MS表型和健康受试者 (HS) 方面存在重大知识差距.

研究的目的:

调查不同血清代谢物概况是否可以区分RRMS,PPMS和HS.
确定与不同多发性硬化症疾病过程相关的特定代谢途径和生物标志物.

主要方法:

使用2D GC-GC-MS平台的全球非向代谢学被采用.
从41名RRMS患者,31名PPMS患者和91名HS患者的血清样本中分析了235种代谢物.
进行了统计分析和途径丰富分析 (MetaboAnalyst,Qiagen IPA),以确定显著差异和相关的生物途径.

主要成果:

在RRMS和HS (22个代谢物) 之间以及PPMS和HS (28个代谢物) 之间观察到代谢物概况的显著变化.
与HS相比,包括银河糖代谢和氨基糖代谢在内的四种代谢途径通常都在RRMS和PPMS中富含.
某些特定的代谢物,如氨酸,甲酸,α-ketoisocaproic酸和糖与PPMS中神经退行和中枢神经系统炎症有关.

结论:

改变的血清代谢物概况可以作为潜在的生物标志物来区分MS疾病过程 (RRMS,PPMS) 和健康个体.
已识别的代谢指纹为开发诊断工具和代谢物面板提供了潜在的潜力,用于MS等进展性自身免疫性疾病.
对SULF2和ITGB1BP1等特定上游调节物的进一步研究可能会阐明MS的发病因子.

关键词:

在 GC-GC-MS 中使用.这是一个PPMSPPMS.在RRMS中使用RRMS.代谢生物组的代谢生物组多发性硬化症多发性硬化症

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